Mazzarelli J M, Atkins G B, Geisberg J V, Ricciardi R P
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Oncogene. 1995 Nov 2;11(9):1859-64.
A function shared by the adenovirus E1A, papillomavirus E7 and SV40 TAg oncoproteins is their ability to interfere with normal cell growth by interacting with members of the retinoblastoma protein family. In this study, we show that each of these oncoproteins can also bind to the 921 amino acid TBP-associated factor-110 (TAF-110). The significance of the binding is underscored by the observation that each oncoprotein binds to the same 77 amino acid carboxyl region of TAF-110. In the case of E1A and TAg, this finding is consistent with their abilities to stimulate transcription initiation, in part, through their known interactions with TBP. While it is not clear whether E7 can also activate promoters through protein:protein interactions with components of the transcription initiation complex, our demonstration that E7 can bind to TAF-110, as well as TBP, suggests that E7 may modulate the expression of specific promoters which could contribute to the pathogenesis of human papillomavirus.
腺病毒E1A、乳头瘤病毒E7和SV40 T抗原癌蛋白的一个共同功能是它们能够通过与视网膜母细胞瘤蛋白家族成员相互作用来干扰正常细胞生长。在本研究中，我们发现这些癌蛋白中的每一种还能与921个氨基酸的TBP相关因子110（TAF-110）结合。每种癌蛋白都与TAF-110相同的77个氨基酸的羧基区域结合，这一观察结果突出了这种结合的重要性。就E1A和T抗原而言，这一发现与它们部分通过与TBP的已知相互作用来刺激转录起始的能力是一致的。虽然尚不清楚E7是否也能通过与转录起始复合物成分的蛋白质-蛋白质相互作用来激活启动子，但我们证明E7能与TAF-110以及TBP结合，这表明E7可能调节特定启动子的表达，这可能有助于人乳头瘤病毒的发病机制。
