Fischer R G, Edwardsson S, Klinge B
Department of Periodontology, Centre for Oral Health Sciences, Lund University, Malmö, Sweden.
Oral Microbiol Immunol. 1994 Feb;9(1):40-9. doi: 10.1111/j.1399-302x.1994.tb00213.x.
The aim of this study was to describe, in the domestic ferret, the composition of the normal gingival sulcus and the near related mucosa microflora and the shift in the composition during an acute periodontal breakdown induced by the placement of ligatures. Five animals were used. At the start of the experiment (day 0) and before the placement of the ligatures, 2 microbiological samples were obtained from the gingival sulcus and the related mucosa at the mid-buccal surface of the right upper P4. The same sites were sampled on the left side, serving as control sites. On day 28, new microbiological samples were taken from the same 4 sites. The samples were analyzed with darkfield microscopy and with viable counts. In the darkfield analysis, on day 0, the predominant bacterial morphotype was cocci, ranging from 88-92%. The viable counts showed that nearly 100% of the total cultivable flora was composed of facultative anaerobic gram-negative and gram-positive rods. Pasteurella spp., Corynebacterium spp. and Rothia spp. were the major components of these bacterial groups. No anaerobic bacteria were detected. On day 28, cocci decreased to approximately 75% in the experimental gingival sulcus sample, and filaments and rods increased significantly. Spirochetes remained largely unchanged during the experiment. The composition of the experimental site samples changed. There was a decrease in the mean numbers of facultative anaerobic gram-positive rods, and approximately one third of the microflora comprised anaerobic gram-negative rods, mainly Porphyromonas gingivalis and Fusobacterium spp. Although the composition of the microflora of the ferret presented few similarities to that described in dogs and monkeys, the anaerobic portion of the gingival sulcus and oral mucosa microflora related to ligature-induced periodontitis presented microorganisms observed in experimental periodontitis both in dogs and monkeys as well as in human periodontal diseases.
本研究的目的是描述家养雪貂正常龈沟及相关黏膜微生物群落的组成，以及在结扎诱导的急性牙周破坏期间其组成的变化。使用了5只动物。在实验开始时（第0天）且在结扎前，从右上第四前臼齿颊面中部的龈沟和相关黏膜获取2份微生物样本。左侧相同部位作为对照部位进行采样。在第28天，从相同的4个部位采集新的微生物样本。样本采用暗视野显微镜和活菌计数法进行分析。在暗视野分析中，第0天，主要的细菌形态类型为球菌，占88 - 92%。活菌计数显示，几乎100%的可培养菌群由兼性厌氧革兰氏阴性菌和革兰氏阳性杆菌组成。巴斯德菌属、棒状杆菌属和罗氏菌属是这些细菌类群的主要成分。未检测到厌氧菌。在第28天，实验龈沟样本中的球菌减少至约75%，丝状菌和杆菌显著增加。螺旋体在实验期间基本保持不变。实验部位样本的组成发生了变化。兼性厌氧革兰氏阳性杆菌的平均数量减少，约三分之一的微生物群落由厌氧革兰氏阴性杆菌组成，主要是牙龈卟啉单胞菌和梭杆菌属。尽管雪貂微生物群落的组成与狗和猴子中描述的组成几乎没有相似之处，但与结扎诱导的牙周炎相关的龈沟和口腔黏膜微生物群落的厌氧部分呈现出在狗和猴子的实验性牙周炎以及人类牙周疾病中观察到的微生物。
