人tRNA（Leu）的识别元件：体外将人tRNA（Ser）转化为亮氨酸受体的结构要求。
Identity elements of human tRNA(Leu): structural requirements for converting human tRNA(Ser) into a leucine acceptor in vitro.
作者信息
Breitschopf K, Achsel T, Busch K, Gross H J
机构信息
Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
出版信息
Nucleic Acids Res. 1995 Sep 25;23(18):3633-7. doi: 10.1093/nar/23.18.3633.
Abstract
We have previously shown that the exchange of the discriminator base A73 of human tRNA(Leu) for G is alone sufficient to achieve complete loss of leucine acceptance and to create an efficient serine acceptor. The reverse identity switch, however, which was studied using T7 RNA polymerase transcripts of in vitro mutagenized tRNA genes, reveals a far more complex pattern of identity elements for tRNA(Leu). Introduction of the following tRNA(Leu)-specific structures is necessary to transform human tRNA(Ser) into an efficient leucine acceptor: the discriminator base A73, the base pairs C3:G70, A4:U69 and G5:C68 of the acceptor stem, C20a of the DHU loop and the long extra arm. In contrast to tRNA(Ser), human tRNA(Leu) identity requires both the sequence and the correct orientation of the long extra arm, whereas only its orientation is essential for serine identity.
摘要
我们之前已经表明，将人tRNA(Leu)的鉴别碱基A73替换为G，就足以完全丧失亮氨酸接纳能力，并产生一个高效的丝氨酸接纳体。然而，使用体外诱变的tRNA基因的T7 RNA聚合酶转录本进行研究的反向身份转换，揭示了tRNA(Leu)的身份元件模式要复杂得多。要将人tRNA(Ser)转化为高效的亮氨酸接纳体，需要引入以下tRNA(Leu)特异性结构：鉴别碱基A73、接纳茎的碱基对C3:G70、A4:U69和G5:C68、DHU环的C20a以及长额外臂。与tRNA(Ser)不同，人tRNA(Leu)的身份既需要长额外臂的序列，也需要其正确方向，而对于丝氨酸身份来说，只有其方向是至关重要的。
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