Hoffman D W, White S W
Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.
Nucleic Acids Res. 1995 Oct 25;23(20):4058-65. doi: 10.1093/nar/23.20.4058.
Transcription of lentiviral DNA in the host cell is regulated by an interaction between the viral TAR RNA stem-loop and the viral Tat protein. Here we present a model of the three-dimensional structure of the TAR RNA stem-loop of the equine infectious anemia virus (EIAV), derived from two- and three-dimensional NMR data. This 25 nucleotide RNA consists of an A-form helical stem capped by two U-G base pairs and a four-nucleotide loop. Two loop cytidines are stacked into the loop interior and likely form a non-Watson-Crick C-C base-pair. The two nucleotides at the top of the loop, U13 and G14, appear to be excluded from the interior of the loop and solvent exposed. It is significant that now for the EIAV TAR-Tat system, three-dimensional structures are now known for both the RNA and protein components.
慢病毒DNA在宿主细胞中的转录受病毒TAR RNA茎环与病毒Tat蛋白之间相互作用的调控。在此，我们基于二维和三维核磁共振数据，展示了马传染性贫血病毒（EIAV）的TAR RNA茎环的三维结构模型。这种25个核苷酸的RNA由一个A形式的螺旋茎组成，该螺旋茎由两个U-G碱基对封端，并带有一个四核苷酸环。两个环胞嘧啶堆积在环内部，可能形成一个非沃森-克里克C-C碱基对。环顶部的两个核苷酸U13和G14似乎被排除在环内部且暴露于溶剂中。重要的是，现在对于EIAV TAR-Tat系统，RNA和蛋白质成分的三维结构均已为人所知。
