Wijmenga S S, Heus H A, Werten B, van der Marel G A, van Boom J H, Hilbers C W
Nijmegen SON Research Center for Molecular Design, Structure and Synthesis, HF-NMR Facility, University of Nijmegen, The Netherlands.
J Magn Reson B. 1994 Feb;103(2):134-41. doi: 10.1006/jmrb.1994.1021.
The application of 3D TOCSY-NOESY to the analysis of RNA is presented, using a TOCSY-NOESY spectrum of the RNA duplex r(5'GGGCUGAAGCCU'). It is shown that for RNA molecules, 3D spectra can be obtained with a digital resolution comparable to that obtained for 2D NMR with full spectral information. The improvement in assignment over 2D methods is shown and discussed on the basis of an assignment strategy presented earlier. A simple and straightforward method for determining sugar puckers and gamma backbone torsion angles is presented, which is derived from an analysis of cross-peak intensities originating from the TOCSY coherence transfer among sugar protons and H5'/5" protons. The stereospecific assignment of the H5'/5" resonances in 3D TOCSY-NOESY spectra is also discussed.
本文介绍了3D TOCSY - NOESY在RNA分析中的应用，使用了RNA双链体r(5'GGGCUGAAGCCU')的TOCSY - NOESY谱。结果表明，对于RNA分子，可以获得具有与二维核磁共振（2D NMR）相当的数字分辨率的三维谱，且包含完整的光谱信息。基于之前提出的归属策略，展示并讨论了与二维方法相比在归属方面的改进。提出了一种简单直接的方法来确定糖环构象和γ骨架扭转角，该方法源于对糖质子与H5'/5"质子之间通过TOCSY相干转移产生的交叉峰强度的分析。还讨论了3D TOCSY - NOESY谱中H5'/5"共振的立体专一性归属。
