Baines R A, Thompson K S, Rayne R C, Bacon J P
Sussex Centre for Neuroscience, University of Sussex, Brighton, UK.
Peptides. 1995;16(5):799-807. doi: 10.1016/0196-9781(95)00038-l.
Isolated cell bodies of the locust vasopressin-like immunoreactive (VPLI) neurons, analyzed by HPLC separation and radioimmune assay, contain three arginine vasopressin-like peptides: a previously identified monomer (Fl, Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2) and its antiparallel homodimer (F2), but also the previously unreported parallel homodimer (PDm). VPLI neuron activity significantly reduces the level of cAMP in the CNS. Of the three synthetic peptides, only the monomer (F1, 10(-8) and 10(-6) M) is capable of inhibiting a forskolin-stimulated increase in cAMP in isolated neural membranes. The antiparallel (F2) and parallel dimers (PDm) of this peptide have no effect on this second messenger.
通过高效液相色谱分离和放射免疫分析对蝗虫类血管加压素免疫反应性（VPLI）神经元的分离细胞体进行分析，结果显示其含有三种精氨酸血管加压素样肽：一种先前已鉴定的单体（F1，Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2）及其反平行同二聚体（F2），还有先前未报道的平行同二聚体（PDm）。VPLI神经元活性显著降低中枢神经系统中cAMP的水平。在这三种合成肽中，只有单体（F1，10^(-8)和10^(-6) M）能够抑制佛司可林刺激的分离神经膜中cAMP的增加。该肽的反平行（F2）和平行二聚体（PDm）对这种第二信使没有影响。
