内皮素转化酶的荧光偏振测定法。
Fluorescence polarization assay for endothelin-converting enzymes.
作者信息
Hazan S, Fishov I, Zamai M, Caiolfa V R, Parola A H
机构信息
Department of Chemistry, Ben Gurion University of the Negev, Beer-Sheva, Israel.
出版信息
Peptides. 1995;16(5):833-6. doi: 10.1016/0196-9781(95)00032-f.
PMID:7479323
Abstract
The conversion of big endothelin to endothelin by alpha-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.
摘要
通过跟踪大内皮素单一色氨酸的荧光偏振来测定α-糜蛋白酶将大内皮素转化为内皮素的过程。这为寻找天然内皮素转化酶提供了一种新颖、简单、快速且灵敏的鉴定方法。
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