Pierson P M, Guibbolini M E, Mayer-Gostan N, Lahlou B
Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS URA 1938, Faculté des Sciences, Nice, France.
Peptides. 1995;16(5):859-65. doi: 10.1016/0196-9781(95)00058-r.
The fish neurohypophysial hormones arginine vasotocin (AVT) and isotocin (IT) were measured for the first time by ELISAs (in comparison with other techniques) in plasma and hypophysis of rainbow trout adapted stepwise from freshwater (FW) to seawater (SW). AVT concentrations were higher than IT in plasma and, conversely, lower in hypophysis. No difference appeared between FW and SW conditions, but plasma hormone concentrations fell when FW fish were moved to 1/3 SW and increased progressively when fish were moved from 1/3 SW to SW. Peptide values obtained in 1/3 SW may correspond to the lowest osmoregulatory constraints occurring in an isosmotic medium in comparison to FW or full SW. The data suggest that storage and/or release of AVT and IT differ, but vary in a similar way with external salinity, and that these peptides should play a role in teleost fish osmoregulation.
采用酶联免疫吸附测定法（ELISA，与其他技术作比较）首次测定了逐步从淡水（FW）适应到海水（SW）的虹鳟血浆和垂体中鱼类神经垂体激素精氨酸加压催产素（AVT）和异催产素（IT）的含量。血浆中AVT的浓度高于IT，相反，垂体中AVT的浓度低于IT。淡水和海水环境下未出现差异，但当淡水鱼转移至1/3海水环境时，血浆激素浓度下降，而当鱼从1/3海水环境转移至海水环境时，血浆激素浓度则逐渐升高。与淡水或全海水环境相比，在1/3海水环境中获得的肽值可能对应于等渗介质中出现的最低渗透调节限制。数据表明，AVT和IT的储存和/或释放存在差异，但随外部盐度以类似方式变化，并且这些肽应在硬骨鱼类渗透调节中发挥作用。
