Warne J M, Hazon N, Rankin J C, Balment R J
School of Biological Sciences, University of Manchester, United Kingdom.
Gen Comp Endocrinol. 1994 Dec;96(3):438-44. doi: 10.1006/gcen.1994.1200.
A specific radioimmunoassay was developed and characterized for the measurement of arginine vasotocin (AVT) in teleost fish. Specificity of the antibody for AVT was demonstrated by parallelism of a series of AVT standards with serially diluted pituitary and plasma extracts. Crossreactivity of the antibody with the other teleost neurohypophysial peptide, isotocin, was less than 1% and the sensitivity of the assay was 0.24 fmol/assay tube. AVT was extracted from plasma by reverse-phase liquid chromatography [efficiency of 87.6 +/- 9.3% (n = 5)] and demonstrated as an effective procedure for plasma volumes ranging from 0.4 to 1.2 ml. Plasma AVT concentrations measured in a range of euryhaline and stenohaline teleost fish were between 10(-12) and 2 x 10(-11) M (1-20 pg/ml). There were no consistent differences between plasma AVT levels in euryhaline fish (flounder, trout, and eel) adapted to fresh water (FW) and sea water (SW). In flounder, pituitary AVT levels in FW- and SW-adapted fish were also similar.
开发并鉴定了一种用于测量硬骨鱼中精氨酸加压催产素（AVT）的特异性放射免疫分析法。通过一系列AVT标准品与连续稀释的垂体和血浆提取物的平行性，证明了抗体对AVT的特异性。该抗体与另一种硬骨鱼神经垂体肽异催产素的交叉反应率小于1%，该分析方法的灵敏度为0.24 fmol/分析管。采用反相液相色谱法从血浆中提取AVT[效率为87.6±9.3%（n = 5）]，并证明该方法对0.4至1.2 ml的血浆量有效。在一系列广盐性和狭盐性硬骨鱼中测得的血浆AVT浓度在10⁻¹²至2×10⁻¹¹ M（1 - 20 pg/ml）之间。适应淡水（FW）和海水（SW）的广盐性鱼类（比目鱼、鳟鱼和鳗鱼）的血浆AVT水平之间没有一致的差异。在比目鱼中，适应淡水和海水的鱼的垂体AVT水平也相似。
