Mackenstedt U, Gauer M, Fuchs P, Zapf F, Schein E, Mehlhorn H
Lehrstuhl für Spezielle Zoologie und Parasitologie, Ruhr-Universität Bochum, Germany.
Parasitol Res. 1995;81(7):595-604. doi: 10.1007/BF00932027.
The relative DNA levels of different developmental stages of Babesia bigemina and B. canis were measured by cytophotometry using the fluorochrome Hoechst 33258 as a staining dye. The DNA measurements provided direct proof of sexual reproduction, i.e., fusion of gametes resulting in the formation of zygotes followed by a meiotic division. Both Babesia species are considered to be typical members of the genus Babesia; however, the DNA measurements revealed important differences in the life cycle of these parasites, indicating that Babesia species are not characterized by a life cycle, which is specific for this genus.
使用荧光染料Hoechst 33258通过细胞光度法测量了双芽巴贝斯虫和犬巴贝斯虫不同发育阶段的相对DNA水平。DNA测量为有性生殖提供了直接证据，即配子融合导致合子形成，随后进行减数分裂。这两种巴贝斯虫都被认为是巴贝斯属的典型成员；然而，DNA测量揭示了这些寄生虫生命周期中的重要差异，表明巴贝斯虫物种并不具有该属特有的生命周期特征。
