Faletti A, Viggiano J M, Gimeno M A
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Prostaglandins. 1995 Feb;49(2):93-103. doi: 10.1016/0090-6980(94)00006-i.
The aim of the present study was to explore the action of exogenous beta-endorphin on the number of oocytes ovulated and on prostaglandin (PG) production in ovaries isolated from pregnant mare's serum gonadotropin/human chorionic gonadotropin(PMSG/hCG)-primed immature rats. An intrabursal injection of the opioid (0.084 microgram) was given 4 hours after hCG and the number of oocytes within the oviducts on the following morning was reduced (P < 0.05). The same effect was also attained with an intraperitoneal (IP) injection (0.5 microgram). The time course of PG synthesis was quantified in ovaries of rats treated with an IP injection. Eight hours after hCG, prostaglandin content increased (P < 0.01) and remained high until 12 hours after hCG (P < 0.001). This increase was inhibited by the in vivo treatment with beta-endorphin. On isolated ovaries, beta-endorphin (10(-8) M) had a clear inhibitory action on prostaglandin production. beta-Endorphin's effect on prostaglandin synthesis in the ovaries is of importance in the ovulatory process. The possible physiological role of beta-endorphin merits further investigation.
本研究的目的是探讨外源性β-内啡肽对从孕马血清促性腺激素/人绒毛膜促性腺激素（PMSG/hCG）预处理的未成熟大鼠分离出的卵巢中排卵的卵母细胞数量以及前列腺素（PG）产生的作用。在hCG注射4小时后进行卵巢囊内注射阿片样物质（0.084微克），次日上午输卵管内的卵母细胞数量减少（P<0.05）。腹腔内（IP）注射（0.5微克）也能达到相同效果。对接受IP注射治疗的大鼠卵巢中PG合成的时间进程进行了定量分析。hCG注射8小时后，前列腺素含量增加（P<0.01），并一直保持较高水平直到hCG注射后12小时（P<0.001）。这种增加受到β-内啡肽体内治疗的抑制。在分离的卵巢上，β-内啡肽（10^(-8) M）对前列腺素产生有明显的抑制作用。β-内啡肽对卵巢中前列腺素合成的作用在排卵过程中具有重要意义。β-内啡肽可能的生理作用值得进一步研究。
