Satoh K, Mitsuhashi N, Kinoshita K, Kawai Y, Maeda R, Sakamoto S
Prostaglandins Med. 1981 Jun;6(6):589-600. doi: 10.1016/0161-4630(81)90120-8.
Biosynthesis of prostaglandins (PGs) from 14C-arachidonic acid was studied using homogenates of the ovaries from immature rats. In ascending order of metabolizing potency were, the ovaries from untreated rats, from rats treated with pregnant mare's serum gonadotropin (PMS), and from PMS-human chorionic gonadotropin (hCG) treated rats. Among the radioactive metabolites extracted, PGE2 and 6-keto PGF1 were purified and identified by silicic acid column-, thin layer-, reversed phase partition chromatographies, and radiogaschromatography. Production of PGE2 and 6-keto PGF1 was observed in homogenates of the ovaries of intact and PMS-hCG treated rats at conversion rates of 0.72; 0.43% and 7.62; 2.31%, but not by PMS treated rat ovaries. Treatment with PMS-hCG activated metabolism of arachidonic acid into radioactive metabolites including PGE2 and 6-keto PGF1 to a large extent. Accordingly, it is concluded that luteinizing hormone and hCG play a significant role in the biosynthesis of PGs by the rat ovarian follicle.
利用未成熟大鼠的卵巢匀浆研究了从14C-花生四烯酸生物合成前列腺素（PGs）的过程。代谢能力由低到高依次为：未经处理的大鼠卵巢、用孕马血清促性腺激素（PMS）处理的大鼠卵巢以及用PMS-人绒毛膜促性腺激素（hCG）处理的大鼠卵巢。在提取的放射性代谢产物中，通过硅酸柱色谱、薄层色谱、反相分配色谱和放射性气相色谱对PGE2和6-酮-PGF1进行了纯化和鉴定。在完整大鼠和PMS-hCG处理大鼠的卵巢匀浆中观察到了PGE2和6-酮-PGF1的生成，转化率分别为0.72%；0.43%和7.62%；2.31%，但PMS处理的大鼠卵巢未观察到。PMS-hCG处理在很大程度上激活了花生四烯酸向包括PGE2和6-酮-PGF1在内的放射性代谢产物的代谢。因此，得出结论：促黄体生成素和hCG在大鼠卵巢卵泡PGs的生物合成中起重要作用。
