Saha B, Bandyopadhyay D, Roy S
Indian Institute of Chemical Biology, Jadavpur, Calcutta, India.
Scand J Immunol. 1995 Nov;42(5):540-6. doi: 10.1111/j.1365-3083.1995.tb03693.x.
The evolution of cells of the monocyte-macrophage lineage (MML cells) in the spleen of Leishmania donovani (LD) infected BALB/c mice was studied. Spleen cells were fractionated on a discontinuous percoll gradient and adherent cells (AC) were purified from fractionated spleen cells by adherence steps that appeared at the interfaces of 25-35%, 35-40%, 40-45% and 45-50% percoll gradients. The AC were characterized as MML cells on the basis of positive staining for non-specific esterase. Adherent cells that appeared at the interfaces of 25-35% and 40-45% were defined as A and C, respectively, and both of them showed extreme variation in a progressive infection. It was observed that A supported parasite replication whereas C remained refractory when infected with LD in vitro. Furthermore, when A cells and C cells were used as antigen-presenting cells to stimulate mixed population of IFN-gamma producing and IL-4 producing T-cells, it was observed that IL-4 and IFN-gamma were the predominating cytokine in the T-cell supernatant, respectively. Both A and C were found to be increased hand-in-hand up to 5 months of infection and from then on A decreased and C increased in their numerical strength (A-C reciprocity). The evolution of A-C reciprocity coincided with the gradual reduction in the parasitaemia in the spleen suggesting that this may contribute to the acquisition of anti-leishmania immunity.
研究了利什曼原虫（LD）感染的BALB/c小鼠脾脏中单核细胞-巨噬细胞谱系（MML细胞）的细胞演变。脾细胞在不连续的 Percoll 梯度上进行分级分离，通过在25%-35%、35%-40%、40%-45%和45%-50% Percoll 梯度界面出现的贴壁步骤，从分级分离的脾细胞中纯化贴壁细胞（AC）。基于非特异性酯酶的阳性染色，将AC鉴定为MML细胞。分别将在25%-35%和40%-45%界面出现的贴壁细胞定义为A和C，在进行性感染中它们都表现出极大的变异性。观察到，在体外感染LD时，A支持寄生虫复制，而C仍然具有抗性。此外，当将A细胞和C细胞用作抗原呈递细胞来刺激产生IFN-γ和产生IL-4的T细胞混合群体时，观察到IL-4和IFN-γ分别是T细胞上清液中的主要细胞因子。发现A和C在感染5个月前均同步增加，从那时起，A的数量减少，C的数量增加（A-C互作）。A-C互作的演变与脾脏中寄生虫血症的逐渐降低相吻合，表明这可能有助于获得抗利什曼原虫免疫力。
