Shev S, Widell A, Bergström T, Hermodsson S, Lindholm A, Norkrans G
Department of Infectious Diseases, Ostra University Hospital, Göteborg, Sweden.
Sex Transm Dis. 1995 Jul-Aug;22(4):210-6. doi: 10.1097/00007435-199507000-00002.
Antibodies against herpes simplex viruses-1 and -2, cytomegalovirus, and syphilis were determined in six heterosexual couples with strong indications of having sexually transmitted hepatitis C virus infection and in 17 other heterosexual couples in which one partner was hepatitis C virus viremic (source partner), but the other had remained hepatitis C virus uninfected (exposed partner).
Antibody testing was done with an enzyme-linked immunosorbent assay. Anti-herpes simplex virus 2 and anti-hepatitis C virus findings were further confirmed by immunoblotting. Hepatitis C virus RNA was determined by polymerase chain reaction and genotyped with type-specific primers.
Five of six anti-hepatitis C virus-positive exposed heterosexual partners without parenteral risk factors, compared with three of 17 anti-hepatitis C virus-negative exposed partners, had antibodies to herpes simplex virus-2. On the other hand, no statistically significant difference was found regarding the frequency of herpes simplex virus-2 seropositivity when source partners in the anti-hepatitis C virus concordant and discordant couples were compared. The presence of antibodies to herpes simplex virus-1, cytomegalovirus, and syphilis did not significantly differ between source or exposed partners in anti-hepatitis C virus concordant and discordant couples, respectively. No predominance of any one hepatitis C virus genotype or liver morphology in couples concordant compared with discordant for anti-hepatitis C virus was found.
The findings support the role of herpes simplex virus-2 in the heterosexual transmission of hepatitis C virus infections, and more specifically an increase in susceptibility to hepatitis C virus infections in exposed heterosexual partners with antibodies to herpes simplex virus-2.
在六对有强烈性传播丙型肝炎病毒感染迹象的异性恋伴侣以及另外17对异性恋伴侣中检测了抗单纯疱疹病毒1型和2型、巨细胞病毒以及梅毒的抗体，在这17对伴侣中，一方为丙型肝炎病毒血症患者（传染源伴侣），而另一方未感染丙型肝炎病毒（暴露伴侣）。
采用酶联免疫吸附测定法进行抗体检测。抗单纯疱疹病毒2型和抗丙型肝炎病毒的检测结果通过免疫印迹法进一步确认。通过聚合酶链反应测定丙型肝炎病毒RNA，并使用型特异性引物进行基因分型。
在六名无肠道外危险因素的抗丙型肝炎病毒阳性的暴露异性恋伴侣中，有五人抗单纯疱疹病毒2型抗体呈阳性，而在17名抗丙型肝炎病毒阴性的暴露伴侣中，有三人呈阳性。另一方面，在比较抗丙型肝炎病毒一致和不一致的伴侣组中的传染源伴侣时，单纯疱疹病毒2型血清阳性频率未发现有统计学意义的差异。抗丙型肝炎病毒一致和不一致的伴侣组中，传染源或暴露伴侣的抗单纯疱疹病毒1型、巨细胞病毒和梅毒抗体的存在情况分别无显著差异。未发现抗丙型肝炎病毒一致与不一致的伴侣组中丙型肝炎病毒基因型或肝脏形态有任何优势。
这些发现支持单纯疱疹病毒2型在丙型肝炎病毒感染异性传播中的作用，更具体地说，支持抗单纯疱疹病毒2型抗体的暴露异性恋伴侣对丙型肝炎病毒感染易感性增加的观点。
