Bernheim N, Wang D, Clement P A
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital, Free University of Brussels, Belgium.
Acta Otorhinolaryngol Belg. 1995;49(3):229-33.
The objective of these experiments was to study the dynamics of the cytologic and rhinomanometric nasal reaction after allergen challenge. For this purpose a micro-suction system was developed to repetitively and atraumatically collect nasal secretions. Outside of the pollen season, four patients allergic to grass pollen were challenged with phosphate buffered saline (PBS) and, two weeks later, with grass pollen. Six control subjects were challenged with PBS only. Repetitive nasal secretion samplings over three days and rhinomanometry (PAR) were performed. Analysis of samplings taken before allergen challenge proved the atraumatic character of the micro-suction and provocation methods. Coupling the obtained dynamic data of nasal cytology and PAR, the following observations could be made: 1) Higher number of eosinophils in patients in the absence of antigen, compared to controls, suggesting a constitutional characteristic of atopy. 2) A close temporal association between cytological and clinical events occurring during the late phase of the allergic reaction. Nasal obstruction seems to be correlated with the influx of eosinophils rather than with their absolute concentration.
这些实验的目的是研究变应原激发后鼻腔细胞学和鼻阻力测量反应的动态变化。为此，开发了一种微量抽吸系统，用于反复且无创地收集鼻分泌物。在花粉季节之外，对4名对草花粉过敏的患者先用磷酸盐缓冲盐水（PBS）进行激发，两周后再用草花粉进行激发。6名对照受试者仅用PBS进行激发。在三天内进行了反复的鼻分泌物采样和鼻阻力测量（PAR）。对变应原激发前采集的样本进行分析，证实了微量抽吸和激发方法的无创性。结合所获得的鼻腔细胞学和PAR的动态数据，可得出以下观察结果：1）与对照组相比，在无抗原情况下患者的嗜酸性粒细胞数量更多，提示特应性的体质特征。2）在过敏反应后期发生的细胞学和临床事件之间存在密切的时间关联。鼻阻塞似乎与嗜酸性粒细胞的流入有关，而非与其绝对浓度有关。
