Takamoto M, Miyazaki N, Ishibashi T, Sugiyama K
Jpn J Exp Med. 1978 Oct;48(5):419-25.
Induction of experimental emphysema by protease was performed with several proteases both in vivo and in vitro to compare the ability of inducing emphysematous change and their elastolytic activity. The following results were obtained. 1) Only elastase and papain have emphysema inducing capacity. Emphysematous changes induced by elastase in vitro were dose dependent. But papain has no genuine elastolytic activity. Nature of the emphysema-inducing capacity of papain remains obscure. 2) Advantages of using the isolated lung for experiment were discussed, especially for the small dose required for enzyme-instillation. Moreover, there is no interferencey by endogeneous enzyme or protease-inhibitors. 3) Guinea pig is useful for the experiemnt of emphysema-induction both in vivo and in vitro. Minimal requirement of elastase is 50 microgram in vitro and 250 microgram for in vivo experiment.
为了比较几种蛋白酶诱导肺气肿改变的能力及其弹性蛋白酶活性，在体内和体外使用多种蛋白酶进行了实验性肺气肿诱导。获得了以下结果。1）只有弹性蛋白酶和木瓜蛋白酶具有诱导肺气肿的能力。弹性蛋白酶在体外诱导的肺气肿改变呈剂量依赖性。但木瓜蛋白酶没有真正的弹性蛋白酶活性。木瓜蛋白酶诱导肺气肿能力的本质仍不清楚。2）讨论了使用离体肺进行实验的优点，特别是酶灌注所需的小剂量。此外，没有内源性酶或蛋白酶抑制剂的干扰。3）豚鼠在体内和体外肺气肿诱导实验中都很有用。体外弹性蛋白酶的最低需求量为50微克，体内实验为250微克。
