Gherardini G, Lundeberg T, Matarasso A, Michaels B, Gazelius B, Brodda-Jansen G, Samuelson U
Department of Plastic Surgery, Karolinska Hospital, Stockholm, Sweden.
Ann Plast Surg. 1995 Aug;35(2):178-83. doi: 10.1097/00000637-199508000-00011.
Vasoconstriction, as a result of mechanical manipulation of blood vessels during microsurgery, may produce a decrease in blood supply and endanger flap viability. A study was undertaken to determine the effects of the topical vasodilator calcitonin gene-related peptide on the microcirculation of flaps after mechanically induced ischemia. A neurovascular island flap based on the superficial epigastric vessels was raised in 42 rats. Blood cell flux in the flap was recorded continuously with a laser Doppler flux meter. The feeding artery was pinched to induce vasospasm, and different concentrations of calcitonin gene-related peptide (10(-7), 10(-8), 10(-9), 10(-10) mol) or a control of sodium chloride 0.9% was applied topically to relieve the ischemia. Results showed that calcitonin gene-related peptide at a concentration of 10(-7) mol significantly shortened the time to reach 50% of the original blood cell flux values (270 +/- 123 seconds) and significantly increased the number of flaps in which the blood cell flux values were restored to prestress levels within 30 minutes. The data support the conclusion that, in this model, topical calcitonin gene-related peptide at the concentration of 10(-7) mol was effective in promoting recovery of the microcirculation after mechanically induced ischemia, without the adverse effects associated with other commonly used vasodilators.
在显微外科手术中，由于对血管进行机械操作而导致的血管收缩，可能会使血液供应减少并危及皮瓣的存活能力。本研究旨在确定局部应用血管扩张剂降钙素基因相关肽对机械性诱导缺血后皮瓣微循环的影响。在42只大鼠身上掀起了以腹壁浅血管为蒂的神经血管岛状皮瓣。用激光多普勒血流仪连续记录皮瓣中的血细胞流量。夹闭供血动脉以诱导血管痉挛，并局部应用不同浓度的降钙素基因相关肽（10⁻⁷、10⁻⁸、10⁻⁹、10⁻¹⁰mol）或0.9%氯化钠作为对照来缓解缺血。结果显示，浓度为10⁻⁷mol的降钙素基因相关肽显著缩短了达到原始血细胞流量值50%所需的时间（270±123秒），并显著增加了血细胞流量值在30分钟内恢复到应激前水平的皮瓣数量。这些数据支持以下结论：在该模型中，浓度为10⁻⁷mol的局部降钙素基因相关肽可有效促进机械性诱导缺血后微循环的恢复，且无其他常用血管扩张剂相关的不良反应。
