Mohandas N, Greenquist A C, Shohet S B
J Supramol Struct. 1978;9(3):453-8. doi: 10.1002/jss.400090315.
Discocytic human red cells undergo discocyte-echinocyte and discocyte-stomatocyte transformations under the action of a wide variety of lipid-soluble anionic and cationic agents respectively. These shape transformations are explained by the bilayer couple hypothesis of Sheetz and Singer to be the result of preferential distribution of the anionic agents in the outer half of the bilayer and the cationic agents in the inner half of the bilayer. We demonstrate that echinocytogenic effects indeed occur when the naturally occurring phospholipid lysophosphatidylcholine (LPC) is localized in the outer half of the bilayer, and stomatocytogenic effects occur when LPC is in the inner half. However, in contrast to the bilayer couple hypothesis, our results show that simple equivalent membrane surface area expansion on each layer is insufficient to maintain the discocytic shape and there exists a differential concentration effect of LPC on the two halves of the bilayer.
盘状的人类红细胞分别在多种脂溶性阴离子和阳离子试剂的作用下经历盘状-棘状红细胞和盘状-口形红细胞转变。根据希茨和辛格的双层偶联假说，这些形状转变被解释为阴离子试剂优先分布在双层外半部分，阳离子试剂优先分布在双层内半部分的结果。我们证明，当天然存在的磷脂溶血磷脂酰胆碱（LPC）位于双层外半部分时，确实会产生棘状红细胞生成效应，而当LPC位于内半部分时，会产生口形红细胞生成效应。然而，与双层偶联假说相反，我们的结果表明，每层简单的等效膜表面积扩展不足以维持盘状形状，并且LPC对双层的两半存在差异浓度效应。
