Jebb A H, Woolsey T A
Stain Technol. 1977 Nov;52(6):315-8. doi: 10.3109/10520297709116805.
A simple and rapid method for demonstrating myelinated nerve fibers in frozen sections of the central and peripheral nervous system is described. Material fixed by perfusion with mixed aldehydes gives the best results but the method also works on specimens fixed by immersion in formaldehyde. Frozen sections varying in thickness from 15-50 micron are mounted on slides subbed with chrome alum-gelatin. After hydration (60-120 min), sections are mordanted (20-40 min) in 2.5% iron alum and rinsed briefly in three changes of distilled H2O (total 2 min). Staining is for 60-180 min in 20 cc freshly made 10% alcoholic hematoxylin diluted with 165 cc distilled H2O to which 15 cc saturated Li2CO3 is added. The sections are washed in distilled H2O (5-15 min) and dehydrated in graded alcohols without differentiation in mordant, and covered. Myelin stains a dark blue-purple against a light grey background. Fiber tracts, as well as individual myelinated fibers, are clearly demonstrated.
本文描述了一种用于显示中枢和周围神经系统冰冻切片中有髓神经纤维的简单快速方法。用混合醛灌注固定的材料效果最佳，但该方法也适用于用甲醛浸泡固定的标本。将厚度为15 - 50微米的冰冻切片安装在涂有铬明矾 - 明胶的载玻片上。水化（60 - 120分钟）后，切片在2.5%铁明矾中媒染（20 - 40分钟），并在三次蒸馏水更换中短暂冲洗（共2分钟）。在20毫升新制备的10%酒精苏木精中染色60 - 180分钟，该苏木精用165毫升蒸馏水稀释，并加入15毫升饱和碳酸锂。切片在蒸馏水中洗涤（5 - 15分钟），在梯度酒精中脱水，无需在媒染剂中分化，然后封片。髓磷脂在浅灰色背景下染成深蓝色 - 紫色。纤维束以及单个有髓纤维都能清晰显示。
