Hutchins B, Weber J T
J Neurosci Methods. 1983 Mar;7(3):289-94. doi: 10.1016/0165-0270(83)90012-2.
A protocol has been established for the staining of myelin in frozen sections. While the new method is relatively fast and simple, it eliminates the problems routinely encountered with myelin stains such as blotchiness and uneven staining. Modifications were introduced into the procedures in order to obtain excellent fiber staining results on a wide variety of tissue.
已建立一种用于冰冻切片中髓磷脂染色的方案。虽然新方法相对快速且简单,但它消除了髓磷脂染色中经常遇到的问题,如斑点和染色不均匀。为了在各种组织上获得出色的纤维染色结果,对程序进行了修改。