Zingeser M R, McNulty W P
Stain Technol. 1977 Nov;52(6):327-30. doi: 10.3109/10520297709116808.
The transparent properties of the embedding agent glycol methacrylate facilitate orientation of the complex embryonic craniofacial region. This technique allows for consistent and reproducible section-to-topography orientation. We find it to be a valuable adjunct for envisioning three-dimensional relationships. The contrast of external features of the embryo is enhanced when stained lightly with hematoxylin prior to embedding. The craniofacial region of the embedded embryo is removed with a fine surgical saw and reimbedded. Section-to-topography relationships are readily monitored and documented photographically. Furthermore, it is possible to "preview" sections for symmetry and other considerations of orientation by viewing the cutting face of the block under oblique illumination. A relief image of structures is then visible.
包埋剂甲基丙烯酸乙二醇酯的透明特性有助于复杂胚胎颅面部区域的定向。该技术可实现切片与地形的一致且可重复的定向。我们发现它是一种用于设想三维关系的有价值的辅助手段。在包埋前用苏木精轻度染色时，胚胎外部特征的对比度会增强。用精细手术锯切除包埋胚胎的颅面部区域并重新包埋。切片与地形的关系很容易监测并通过拍照记录下来。此外，通过在斜射光下观察包埋块的切割面，可以“预览”切片的对称性和其他定向因素。然后可以看到结构的浮雕图像。
