一种通过聚合酶链反应（PCR）直接从食品中检测单核细胞增生李斯特菌的新方法。
A new method for direct detection of Listeria monocytogenes from foods by PCR.
作者信息
Makino S, Okada Y, Maruyama T
机构信息
Department of Veterinary Public Health, Institute of Public Health, Tokyo, Japan.
出版信息
Appl Environ Microbiol. 1995 Oct;61(10):3745-7. doi: 10.1128/aem.61.10.3745-3747.1995.
Abstract
The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.
摘要
在存在Na1的情况下通过乙醇沉淀制备DNA，用于直接检测每0.5克样品中10(3) CFU的单核细胞增生李斯特菌。此方法可直接从软奶酪和碎肉等食品中制备出适用于PCR的DNA。
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