胶原蛋白对检测产气荚膜梭菌的聚合酶链反应的抑制作用。
Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens.
作者信息
Kim S, Labbe R G, Ryu S
机构信息
Department of Food Science and Technology, Seoul National University, Suwon, Korea.
出版信息
Appl Environ Microbiol. 2000 Mar;66(3):1213-5. doi: 10.1128/AEM.66.3.1213-1215.2000.
Abstract
It is essential to identify specific food components that inhibit PCR in order to increase the sensitivity of the PCR method for rapid detection of pathogens contaminating a food. We found that collagen, a major component of several foods, inhibited PCR. The inhibitory action of collagen on PCR could be partially reversed by adjusting the concentration of magnesium ion in the reaction mixture and by the use of various DNA extraction methods to remove the collagen from the DNA. Also, the source of thermostable DNA polymerase was affected by the presence of collagen. These results suggest the need to optimize the extraction and assay conditions for rapid detection of enterotoxigenic Clostridium perfringens by PCR with respect to the kind of food being analyzed.
摘要
为提高聚合酶链反应（PCR）方法对污染食品中病原体快速检测的灵敏度，识别抑制PCR的特定食品成分至关重要。我们发现，胶原蛋白作为几种食品的主要成分，会抑制PCR。通过调整反应混合物中镁离子的浓度以及使用各种DNA提取方法从DNA中去除胶原蛋白，可部分逆转胶原蛋白对PCR的抑制作用。此外，胶原蛋白的存在会影响热稳定DNA聚合酶的来源。这些结果表明，就所分析食品的种类而言，需要优化通过PCR快速检测产肠毒素型产气荚膜梭菌的提取和检测条件。
相似文献
1
Inhibitory effects of collagen on the PCR for detection of Clostridium perfringens.胶原蛋白对检测产气荚膜梭菌的聚合酶链反应的抑制作用。
Appl Environ Microbiol. 2000 Mar;66(3):1213-5. doi: 10.1128/AEM.66.3.1213-1215.2000.
2
Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test.采用双重聚合酶链反应和玻片乳胶凝集试验检测食品和粪便样本中的产肠毒素性产气荚膜梭菌。
Appl Environ Microbiol. 1997 Nov;63(11):4232-6. doi: 10.1128/aem.63.11.4232-4236.1997.
3
Controlled multiplex PCR of enterotoxigenic Clostridium perfringens strains in food samples.食品样本中产肠毒素性产气荚膜梭菌菌株的对照多重聚合酶链式反应
Mol Cell Probes. 1998 Dec;12(6):359-65. doi: 10.1006/mcpr.1998.0196.
4
A 16S rDNA-based PCR method for rapid and specific detection of Clostridium perfringens in food.一种基于16S rDNA的PCR方法，用于快速、特异性检测食品中的产气荚膜梭菌。
Mol Cell Probes. 1994 Apr;8(2):131-7. doi: 10.1006/mcpr.1994.1018.
5
Rapid detection of enterotoxigenic clostridium perfringens by real-time fluorescence resonance energy transfer PCR.
J Food Prot. 2006 Jun;69(6):1347-53. doi: 10.4315/0362-028x-69.6.1347.
6
Multiplex PCR assay for detection of Clostridium perfringens in feces and intestinal contents of pigs and in swine feed.用于检测猪粪便、肠道内容物及猪饲料中产气荚膜梭菌的多重聚合酶链反应检测法。
Vet Microbiol. 1998 Aug 28;63(1):29-38. doi: 10.1016/s0378-1135(98)00229-6.
7
Rapid detection of enterotoxigenic Clostridium perfringens in meat samples using immunomagnetic separation polymerase chain reaction (IMS-PCR).免疫磁珠分离聚合酶链反应快速检测肉样中产肠毒素梭状芽孢杆菌。
J Agric Food Chem. 2010 Jun 23;58(12):7135-40. doi: 10.1021/jf1009654.
8
[Study of real-time PCR assays for rapid detection of food-borne pathogens].
Kansenshogaku Zasshi. 2005 Sep;79(9):644-55. doi: 10.11150/kansenshogakuzasshi1970.79.644.
9
Detection of enterotoxigenic Clostridium perfringens with a duplex PCR.
J Med Microbiol. 2002 Feb;51(2):169-172. doi: 10.1099/0022-1317-51-2-169.
10
Evaluation of PCR and DNA sequencing for direct detection of Clostridium perfringens in the intestinal tract of broilers.聚合酶链反应（PCR）和DNA测序用于直接检测肉鸡肠道中产气荚膜梭菌的评估
Avian Dis. 2009 Sep;53(3):441-8. doi: 10.1637/8569-122608-Reg.1.
引用本文的文献
1
Effect of Proton Pump Inhibitors on Colorectal Cancer.质子泵抑制剂对结直肠癌的影响。
Int J Mol Sci. 2020 May 29;21(11):3877. doi: 10.3390/ijms21113877.
2
Role of Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/ Polyps with Dysplasia.肠毒素在伴异型增生的结直肠无蒂锯齿状腺瘤/息肉中对 YAP 激活的作用。
Int J Mol Sci. 2020 May 28;21(11):3840. doi: 10.3390/ijms21113840.
3
Clostridium perfringens enterotoxin induces claudin-4 to activate YAP in oral squamous cell carcinomas.产气荚膜梭菌肠毒素诱导claudin-4激活口腔鳞状细胞癌中的YAP。
Oncotarget. 2020 Jan 28;11(4):309-321. doi: 10.18632/oncotarget.27424.
4
Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems.基于脂质体的转染增强了非模式线虫系统中的 RNAi 和 CRISPR 介导的诱变。
Sci Rep. 2019 Jan 24;9(1):483. doi: 10.1038/s41598-018-37036-1.
5
Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay.基于链侵入扩增检测法的化脓性链球菌分子检测
Mol Diagn Ther. 2018 Oct;22(5):595-602. doi: 10.1007/s40291-018-0346-8.
6
Monitoring of ochratoxin A and ochratoxin-producing fungi in traditional salami manufactured in Northern Italy.监测意大利北部传统萨拉米香肠中的赭曲霉毒素 A 和产赭曲霉毒素真菌。
Mycotoxin Res. 2018 May;34(2):107-116. doi: 10.1007/s12550-017-0305-y. Epub 2018 Jan 3.
7
Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes.通过靶向α毒素和肠毒素基因的定量实时PCR对人粪便中产气荚膜梭菌进行灵敏定量分析。
BMC Microbiol. 2015 Oct 19;15:219. doi: 10.1186/s12866-015-0561-y.
8
Effective removal of co-purified inhibitors from extracted DNA samples using synchronous coefficient of drag alteration (SCODA) technology.利用同步拖拽系数改变（SCODA）技术有效去除提取 DNA 样本中的共纯化抑制剂。
Int J Legal Med. 2013 Jul;127(4):749-55. doi: 10.1007/s00414-012-0810-7. Epub 2012 Dec 20.
9
Detection of enterotoxigenic Clostridium perfringens in meat samples by using molecular methods.利用分子方法检测肉品中的产肠毒素梭状芽孢杆菌。
Appl Environ Microbiol. 2011 Nov;77(21):7526-32. doi: 10.1128/AEM.06216-11. Epub 2011 Sep 2.
10
Plastic-adherent DNA aptamer-magnetic bead and quantum dot sandwich assay for Campylobacter detection.用于弯曲杆菌检测的塑料粘附DNA适配体-磁珠和量子点夹心分析
J Fluoresc. 2009 May;19(3):427-35. doi: 10.1007/s10895-008-0429-8. Epub 2008 Dec 4.
本文引用的文献
1
Detection of Enterotoxigenic Clostridium perfringens in Raw Beef by Polymerase Chain Reaction.用聚合酶链反应检测生牛肉中的产肠毒素性产气荚膜梭菌
J Food Prot. 1995 Feb;58(2):154-159. doi: 10.4315/0362-028X-58.2.154.
2
Foodborne Disease Outbreaks in the United States, 1973-1987: Pathogens, Vehicles, and Trends.美国1973 - 1987年食源性疾病暴发：病原体、传播媒介及趋势
J Food Prot. 1990 Sep;53(9):804-817. doi: 10.4315/0362-028X-53.9.804.
3
Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples.九种热稳定DNA聚合酶在存在PCR抑制性样本的情况下介导DNA扩增的能力。
Appl Environ Microbiol. 1998 Oct;64(10):3748-53. doi: 10.1128/AEM.64.10.3748-3753.1998.
4
Probes and polymerase chain reaction for detection of food-borne bacterial pathogens.用于检测食源性病原体的探针和聚合酶链反应
Int J Food Microbiol. 1995 Nov;28(1):1-78. doi: 10.1016/0168-1605(94)00159-4.
5
The polymerase chain reaction: applications for the detection of foodborne pathogens.聚合酶链反应：在食源性病原体检测中的应用
Crit Rev Food Sci Nutr. 1996 Jan;36(1-2):123-73. doi: 10.1080/10408399609527721.
6
Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction.通过聚合酶链反应直接检测动物体内的炭疽芽孢杆菌DNA。
J Clin Microbiol. 1993 Mar;31(3):547-51. doi: 10.1128/jcm.31.3.547-551.1993.
7
Rapid detection of food-borne pathogenic bacteria.食源性病原体的快速检测
Annu Rev Microbiol. 1994;48:401-26. doi: 10.1146/annurev.mi.48.100194.002153.
8
Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens.用于鉴定产气荚膜梭菌潜在产肠毒素分离株的蛋白质免疫印迹法与基因检测分析法的比较
J Clin Microbiol. 1994 Oct;32(10):2533-9. doi: 10.1128/jcm.32.10.2533-2539.1994.
9
A new method for direct detection of Listeria monocytogenes from foods by PCR.一种通过聚合酶链反应（PCR）直接从食品中检测单核细胞增生李斯特菌的新方法。
Appl Environ Microbiol. 1995 Oct;61(10):3745-7. doi: 10.1128/aem.61.10.3745-3747.1995.
10
Clostridium perfringens toxins (type A, B, C, D, E).产气荚膜梭菌毒素（A、B、C、D、E型）
Pharmacol Ther. 1980;10(3):617-55. doi: 10.1016/0163-7258(80)90031-5.
Zotero 插件