依赖白细胞介素3的、无唾液酸GM1阳性细胞系，具有天然细胞毒性但无自然杀伤细胞细胞毒性活性。
Interleukin 3-dependent, asialo-GM1-positive cell line with natural cytotoxic but without natural killer cytotoxic activity.
作者信息
Strzadała L, Zioło E, Matuszyk J
机构信息
Laboratory of Cellular Immunology, Polish Academy of Sciences, Wrocław.
出版信息
Arch Immunol Ther Exp (Warsz). 1994;42(3):179-83.
IL-3-dependent cell line (designated as A-3) from adherent spleen cells was developed. The surface phenotype of A-3 cells was analysed by flow cytometry and determined as ASGM1+, FcR+, Ia+. There was no expression of T cell markers (Th1-CD3-CD4-CD8-), B cell marker (surface Ig) as well as macrophage marker Mac-1 antigen. Although A-3 cells expressed ASGM1, they are lacking NK cytotoxic activity in 4 h Cr release assay with YAC-1 targets. In contrast, A-3 cells are endowed with NC cytotoxic activity as shown in 18 h Cr release assay with WEHI-164 target cells.
从贴壁脾细胞中建立了依赖白细胞介素-3的细胞系（命名为A-3）。通过流式细胞术分析了A-3细胞的表面表型，确定为ASGM1+、FcR+、Ia+。未检测到T细胞标志物（Th1-CD3-CD4-CD8-）、B细胞标志物（表面免疫球蛋白）以及巨噬细胞标志物Mac-1抗原的表达。尽管A-3细胞表达ASGM1，但在以YAC-1靶细胞进行的4小时铬释放试验中，它们缺乏自然杀伤细胞的细胞毒性活性。相反，如以WEHI-164靶细胞进行的18小时铬释放试验所示，A-3细胞具有自然细胞毒性活性。
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