Kienzle T E, Henkel J S, Ling J Y, Banks M C, Beers D R, Jones B, Stroop W G
John McClellan Veterans Affairs Medical Center, Little Rock, Arkansas, USA.
Arch Virol. 1995;140(9):1663-75. doi: 10.1007/BF01322540.
EcoRI fragments of herpes simplex virus I (HSV-1) strains H129 and +GC were cloned and the EcoRI and BglII restriction enzyme sites were mapped. Comparison of these enzyme sites with the sequence of HSV-1 strain 17syn+ demonstrated that all EcoRI sites were identical. For H129, the BglII sites were also found to match strain 17syn+ BglII sites. With one exception, the BglII sites in strain +GC also aligned with the strain 17syn+ sequence. The one exception was a missing BglII site from strain +GC located between bases 25,149 and 25,154 in the EcoRI D fragment within the viral deoxyribonuclease gene (UL12). The BglII site represents the first difference to be mapped within HSV-1 strains H129 and +GC which have unique pathobiological properties in animal models of acute and reactivated infections.
克隆了单纯疱疹病毒I型（HSV-1）毒株H129和+GC的EcoRI片段，并绘制了EcoRI和BglII限制性酶切位点图谱。将这些酶切位点与HSV-1毒株17syn+的序列进行比较，结果表明所有EcoRI位点均相同。对于H129，还发现其BglII位点与毒株17syn+的BglII位点匹配。除了一个例外，+GC毒株中的BglII位点也与毒株17syn+的序列一致。这个例外是+GC毒株在病毒脱氧核糖核酸酶基因（UL12）的EcoRI D片段中位于第25149至25154个碱基之间的一个缺失的BglII位点。该BglII位点是在具有急性和再激活感染动物模型中独特病理生物学特性的HSV-1毒株H129和+GC中绘制出的首个差异位点。
