Narita M, Ando Y, Soushi S, Kurata T, Arao Y
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
J Gen Virol. 1998 Mar;79 ( Pt 3):565-72. doi: 10.1099/0022-1317-79-3-565.
Double infection with two interferon (IFN)-sensitive strains of herpes simplex virus (HSV), HSV-1(17syn) and HSV-2(UW268), showed reduced inhibition of virus growth by IFN. Intertypic recombinants with IFN resistance were obtained from the doubly infected cultures. These results indicate that HSV IFN resistance is controlled by at least two genetic regions. Restriction endonuclease analysis demonstrated that the recombinants were similar to HSV-2 in their genomic structure but the BamHI-A, BglII-I and BglII-N fragments of HSV-2 were commonly lost in the recombinants, suggesting that any of these fragments could be associated with HSV-2 IFN resistance. We cloned these fragments and BamHI-E, which overlaps BglII-N, from an IFN-resistant HSV-2 strain, HSV-2(G), and examined each fragment for its ability to rescue IFN resistance of HSV-2(UW268) by co-transfecting with the HSV-2(UW268) genome. Of the HSV-2(G) fragments, only BglII-N increased plating efficiency of progeny viruses in IFN-treated cells. An IFN-resistant HSV-2 clone was obtained from the BglII-N of HSV-2(G) and HSV-2(UW268) genome co-transfected culture, and a part of BglII-N of HSV-2(UW268) was replaced with that of HSV-2(G) in the HSV-2 clone. Thus, it was concluded that one of the HSV regions encoding IFN resistance is located on the BglII-N fragment of HSV-2.
用两种对干扰素（IFN）敏感的单纯疱疹病毒（HSV）毒株，即HSV-1（17syn）和HSV-2（UW268）进行双重感染，结果显示IFN对病毒生长的抑制作用减弱。从双重感染的培养物中获得了具有IFN抗性的异型重组体。这些结果表明，HSV的IFN抗性至少由两个遗传区域控制。限制性内切酶分析表明，重组体在基因组结构上与HSV-2相似，但HSV-2的BamHI-A、BglII-I和BglII-N片段在重组体中通常缺失，这表明这些片段中的任何一个都可能与HSV-2的IFN抗性有关。我们从一株IFN抗性的HSV-2毒株HSV-2（G）中克隆了这些片段以及与BglII-N重叠的BamHI-E，并通过与HSV-2（UW268）基因组共转染，检测每个片段拯救HSV-2（UW268）IFN抗性的能力。在HSV-2（G）的片段中，只有BglII-N提高了IFN处理细胞中子代病毒的铺板效率。从HSV-2（G）的BglII-N与HSV-2（UW268）基因组共转染的培养物中获得了一个IFN抗性的HSV-2克隆，并在该HSV-2克隆中用HSV-2（G）的BglII-N替换了HSV-2（UW268）的部分BglII-N。因此，可以得出结论，HSV编码IFN抗性的区域之一位于HSV-2的BglII-N片段上。
