Redman C A, Schneider P, Mehlert A, Ferguson M A
Department of Biochemistry, University of Dundee, Scotland, U.K.
Biochem J. 1995 Oct 15;311 ( Pt 2)(Pt 2):495-503. doi: 10.1042/bj3110495.
The Phytomonas spp. are trypanosomatid parasites of plants. A polar glycolipid fraction of a Phytomonas sp., isolated from the plant Euphorbia characias and grown in culture, was fractionated into four major glycolipid species (Phy 1-4). The glycolipids were analysed by chemical and enzymic modifications, composition and methylation analyses, electrospray mass spectrometry and microsequencing after HNO2 deamination and NaB3H4 reduction. The water-soluble headgroup of the Phy2 glycolipid was also analysed by 1H NMR. All four glycolipids were shown to be glycoinositol-phospholipids (GIPLs) with phosphatidylinositol (PI) moieties containing the fully saturated alkylacylglycerol lipids 1-O-hexadecyl-2-O-palmitoylglycerol and 1-O-hexadecyl-2-O-stearoylglycerol. The structures of the Phy 1-4 GIPLs are: Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, Glc alpha 1-2(NH2-CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, [formula: see text] Glc alpha 1-2(NH2CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2-CH2CH2-HPO4-)GlcN alpha 1-6PI [formula: see text] and Glc alpha 1-2Glc alpha 1-2(NH2CH2-CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2CH2CH2-HPO4-)-GlcN alpha 1-6PI. [formula: see text] The Phytomonas GIPLs represent a novel series of structures. This is the first description of the chemical structure of cell-surface molecules of this plant pathogen. The Phytomonas GIPLs are compared with those of other trypanosomatid parasites and are discussed with respect to trypanosomatid phylogenetic relationships.
植生滴虫属（Phytomonas spp.）是植物的锥虫类寄生虫。从植物大戟（Euphorbia characias）中分离并在培养条件下生长的一种植生滴虫的极性糖脂组分被分离成四种主要糖脂种类（Phy 1 - 4）。通过化学和酶促修饰、组成和甲基化分析、电喷雾质谱以及在亚硝酸脱氨和硼氢化钠还原后的微测序对这些糖脂进行了分析。还通过1H核磁共振对Phy2糖脂的水溶性头部基团进行了分析。所有四种糖脂均显示为糖肌醇磷脂（GIPLs），其磷脂酰肌醇（PI）部分含有完全饱和的烷基酰基甘油脂质1 - O - 十六烷基 - 2 - O - 棕榈酰甘油和1 - O - 十六烷基 - 2 - O - 硬脂酰甘油。Phy 1 - 4 GIPLs的结构如下：甘露糖α1 - 2甘露糖α1 - 6甘露糖α1 - 4葡糖胺α1 - 6PI、葡萄糖α1 - 2（NH2 - CH2CH2 - HPO4 - ）甘露糖α1 - 2甘露糖α1 - 6甘露糖α1 - 4葡糖胺α1 - 6PI、[化学式：见原文]葡萄糖α1 - 2（NH2CH2CH2 - HPO4 - ）甘露糖α1 - 2甘露糖α1 - 6甘露糖α1 - 4（NH2 - CH2CH2 - HPO4 - ）葡糖胺α1 - 6PI [化学式：见原文]以及葡萄糖α1 - 2葡萄糖α1 - 2（NH2CH2 - CH2 - HPO4 - ）甘露糖α1 - 2甘露糖α1 - 6甘露糖α1 - 4（NH2CH2CH2 - HPO4 - ） - 葡糖胺α1 - 6PI。[化学式：见原文]植生滴虫GIPLs代表了一系列新的结构。这是对这种植物病原体细胞表面分子化学结构的首次描述。将植生滴虫GIPLs与其他锥虫类寄生虫的进行了比较，并就锥虫类的系统发育关系进行了讨论。
