Branquinha M H, Vermelho A B, Almeida I C, Mehlert A, Ferguson M A
Departamento de Microbiologia Geral, Instituto de Microbiologia Professor Paulo de Góes, UFRJ, Rio de Janeiro, Brazil.
Mol Biochem Parasitol. 1999 Jul 30;102(1):179-89. doi: 10.1016/s0166-6851(99)00107-3.
The polar glycoinositol phospholipids (GIPLs) of a Trypanosoma species that belongs to the Schizotrypanum subgenus were purified by reversed-phase and normal-phase liquid chromatography and analysed by negative-ion mode electrospray-mass spectrometry (ES-MS). The phosphatidylinositol moieties were released by nitrous acid deamination and identified as ceramide- and alkylacylglycerol-containing species. The structures of the GIPLs were determined using chemical treatments, sequential exoglycosidase digestions and positive-ion mode ES-MS-MS. All of the GIPLs were based on the same Man alpha1-2Man alpha1-2Man alpha1-6Man alpha1-4(NH2-CH2CH2-HPO3-)GlcN-PI core with single terminal Galf residue substitutions either on the terminal nonreducing Man or on the second alphaMan residue from the inositol and with either ethanolamine phosphate or 2-aminoethylphosphonate on the third alphaMan residue from the inositol. The T. (S.) dionisii GIPLs are compared with those of T. (S.) cruzi, a closely related species of the Schizotrypanum subgenus.
属于裂殖锥虫亚属的一种锥虫的极性糖肌醇磷脂（GIPLs）通过反相和正相液相色谱法进行纯化，并采用负离子模式电喷雾质谱（ES-MS）进行分析。磷脂酰肌醇部分通过亚硝酸脱氨反应释放出来，并鉴定为含神经酰胺和烷基酰基甘油的物质。利用化学处理、外切糖苷酶顺序消化和正离子模式ES-MS-MS确定了GIPLs的结构。所有的GIPLs都基于相同的Manα1-2Manα1-2Manα1-6Manα1-4(NH2-CH2CH2-HPO3-)GlcN-PI核心，在末端非还原Man或距肌醇第二个αMan残基上有单个末端Galf残基取代，并且在距肌醇第三个αMan残基上带有磷酸乙醇胺或2-氨基乙基膦酸。将狄氏锥虫（裂殖锥虫亚属）的GIPLs与克氏锥虫（裂殖锥虫亚属的近缘种）的GIPLs进行了比较。
