Kraus J, Horn G, Zimprich A, Simon T, Mayer P, Höllt V
Universität München, Physiologisches Institut, Germany.
Biochem Biophys Res Commun. 1995 Oct 13;215(2):591-7. doi: 10.1006/bbrc.1995.2505.
The rat mu opioid receptor gene promoter was cloned and characterized. It has a few features in common with the mouse gene, e.g. the lack of a classical TATA box and the fact that several transcriptional start sites are used. The overall homology between the two species is greater than 85%. Functional analysis of the promoter was performed using transient expression of rat mu opioid receptor-reporter gene constructs in the mu opioid receptor expressing cell line SH SY5Y and in non mu opioid receptor expressing cell lines. A promoter region was defined which confers both high basal and TPA and forskolin stimulated reporter gene expression in SH SY5Y cells.
大鼠μ阿片受体基因启动子被克隆并进行了特征分析。它与小鼠基因有一些共同特征，例如缺乏经典的TATA盒以及使用多个转录起始位点。这两个物种之间的总体同源性大于85%。使用大鼠μ阿片受体-报告基因构建体在表达μ阿片受体的细胞系SH SY5Y和不表达μ阿片受体的细胞系中进行瞬时表达，对该启动子进行功能分析。确定了一个启动子区域，该区域在SH SY5Y细胞中赋予高基础水平以及佛波酯（TPA）和福斯高林刺激的报告基因表达。
