Iwase H, Takahashi T, Takatori T, Shimizu T, Aono K, Yamada Y, Iwadate K, Nagao M
Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Oct 24;215(3):945-51. doi: 10.1006/bbrc.1995.2555.
Some monoepoxides of linoleic acid (LA) were converted to monochlorohydrins in low-pH solutions containing chloride ions (Cl-). Conversely, monochlorohydrins of LA were converted to monoepoxides in high-pH solutions. We attempted to determine whether these monochlorohydrins and monoepoxides were produced from LA by the cytochrome-c-H2O2-and/or myeloperoxidase-H2O2-system. The existence of monoepoxides and monochlorohydrins of LA in leukocytes was confirmed by high-performance liquid chromatography (HPLC). Furthermore, leukotoxin in human leukemia cells (THP-1) was stained immunohistochemically by a monoclonal anti-leukotoxin antibody.
一些亚油酸（LA）的单环氧化物在含有氯离子（Cl-）的低pH溶液中转化为单氯醇。相反，LA的单氯醇在高pH溶液中转化为单环氧化物。我们试图确定这些单氯醇和单环氧化物是否由细胞色素c-H2O2-和/或髓过氧化物酶-H2O2-系统从LA产生。通过高效液相色谱法（HPLC）证实了白细胞中LA的单环氧化物和单氯醇的存在。此外，人白血病细胞（THP-1）中的白细胞毒素用单克隆抗白细胞毒素抗体进行免疫组织化学染色。
