Iwase H, Takatori T, Nagao M, Iwadate K, Nakajima M
Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1996 May 6;222(1):83-9. doi: 10.1006/bbrc.1996.0701.
We found that cytochrome c (Cyt c) could oxidize cardiolipin (CL), and detected monoepoxides of linoleic acid (LA) in the fatty acids constituting the oxidized CL. We also found that in the presence of CL and Cyt c, free LA was oxidized and LA monoepoxides were produced. The aim of this study was to elucidate the mechanism of this lipid peroxidation. We concluded that ferric Cyt c produced some radical species from water-soluble oxygen in the presence of CL (CL-Cyt c system) and that radicals oxidized free LA or CL. The CL-Cyt c system may be another LA monoepoxide producing system in the neutrophil and may account for the lipid peroxidation observed in the ischemia-reperfusion-induced cardiac injury.
我们发现细胞色素c（Cyt c）能够氧化心磷脂（CL），并且在构成被氧化CL的脂肪酸中检测到了亚油酸（LA）的单环氧化物。我们还发现，在CL和Cyt c存在的情况下，游离LA会被氧化并生成LA单环氧化物。本研究的目的是阐明这种脂质过氧化的机制。我们得出结论，在CL存在的情况下，三价铁Cyt c会从水溶性氧中产生一些自由基（CL-Cyt c系统），并且这些自由基会氧化游离LA或CL。CL-Cyt c系统可能是中性粒细胞中另一个产生LA单环氧化物的系统，并且可能是缺血再灌注诱导的心脏损伤中观察到的脂质过氧化的原因。
