Kondo K, Ozaki T, Nakamura Y, Sakiyama S
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Biochem Biophys Res Commun. 1995 Nov 2;216(1):209-15. doi: 10.1006/bbrc.1995.2612.
Differential screening-selected gene aberrative in neuroblastoma (DAN) encodes a protein which possesses metal binding motifs. Glutathione S-transferase DAN fusion protein had an ability to bind to Ni(2+)-immobilized affinity resin. Truncation of the C-terminal region including a (HX)n repeat of DAN caused a loss of binding ability to the affinity resin, suggesting that this region is essential for Ni(2+)-binding. DAN produced in cultured rat cells also had an affinity for Ni2+. Cross-linking experiments demonstrated that the C-terminal region might function as a protein-protein interacting domain.
神经母细胞瘤差异筛选选择的基因异常（DAN）编码一种具有金属结合基序的蛋白质。谷胱甘肽S-转移酶DAN融合蛋白具有与固定化镍（2+）亲和树脂结合的能力。DAN C末端区域的截断，包括（HX）n重复序列，导致与亲和树脂结合能力丧失，表明该区域对于镍（2+）结合至关重要。在培养的大鼠细胞中产生的DAN对镍2+也具有亲和力。交联实验表明，C末端区域可能作为蛋白质-蛋白质相互作用结构域发挥作用。
