Tanaka H, Katagiri M, Arima S, Matsuzaki K, Inokoshi J, Omura S
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Nov 2;216(1):291-7. doi: 10.1006/bbrc.1995.2623.
Lactacystin (1.3 microM), a metabolite from an actinomycete, induced the formation of bipolar projections at both sides of the cell body of Neuro 2a cells 1 day after treatment and networks at and after 3 days and enhanced acetylcholinesterase activity (a marker of neuronal differentiation). Thus, the neuronal differentiation was characterized both morphologically and functionally. The experiments with various inhibitors of protein kinases and phosphatases revealed that the protein phosphatase inhibitors calyculin A (0.5 nM) and okadaic acid (0.6 nM) inhibit the formation of bipolar projections at 1 day, but does not inhibit the network formation at and after 3 days.
放线菌代谢产物乳胞素（1.3微摩尔）在处理1天后诱导Neuro 2a细胞胞体两侧形成双极突起，3天及3天后诱导形成网络，并增强乙酰胆碱酯酶活性（神经元分化的标志物）。因此，神经元分化在形态和功能上均有特征表现。使用各种蛋白激酶和磷酸酶抑制剂进行的实验表明，蛋白磷酸酶抑制剂花萼海绵诱癌素A（0.5纳摩尔）和冈田酸（0.6纳摩尔）在1天时抑制双极突起的形成，但在3天及3天后不抑制网络形成。
