Gómez L A, Strasberg Rieber M, Rieber M
I.V.I.C., Tumor Cell Biology Laboratory, Caracas, Venezuela.
Biochem Biophys Res Commun. 1995 Nov 2;216(1):84-9. doi: 10.1006/bbrc.1995.2595.
Malignant transformation is frequently accompanied by changes in the cytoarchitecture of adherent cells, which may be influenced by fluctuations in actin gene expression. We now show that normal melanocytes express a 5 fold higher level of actin mRNA than their melanoma counterparts. Induction of terminal melanogenesis did not increase actin in melanoma cells. However, culture with the thymidine analog, Bromodeoxyuridine, increased actin expression in undifferentiated but not in differentiating melanoma. Cell detachment assays and cell shape comparisons revealed a direct correlation of actin mRNA with increased melanoma cell adhesion rather than with differentiation-mediated suppression of tumor growth.
恶性转化通常伴随着贴壁细胞细胞结构的变化，这可能受肌动蛋白基因表达波动的影响。我们现在表明，正常黑素细胞表达的肌动蛋白mRNA水平比其黑色素瘤对应细胞高5倍。终末黑素生成的诱导并未增加黑色素瘤细胞中的肌动蛋白。然而，用胸苷类似物溴脱氧尿苷培养，可增加未分化黑色素瘤细胞中的肌动蛋白表达，但对分化中的黑色素瘤细胞无效。细胞脱离试验和细胞形状比较显示，肌动蛋白mRNA与黑色素瘤细胞粘附增加直接相关，而非与分化介导的肿瘤生长抑制相关。
