Iwase H, Takatori T, Aono K, Iwadate K, Takahashi M, Nakajima M, Nagao M
Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Nov 13;216(2):483-8. doi: 10.1006/bbrc.1995.2648.
We established an effective monoepoxide-generating system by combining cytochrome-c (Cyt-c) with a hydrogen peroxide (H2O2)-generating system comprising hypoxanthine (HX), xanthine oxidase (XO) and superoxide dismutase (SOD; HX-XO-SOD-Cyt-c system). Using this and the H2O2-Cyt-c system, we proved that monoepoxide production from linoleic acid was due to hydroxy radical formation by the reaction of Cyt-c with H2O2 and not to the formation of other active oxygen species.
我们通过将细胞色素 c（Cyt-c）与由次黄嘌呤（HX）、黄嘌呤氧化酶（XO）和超氧化物歧化酶（SOD）组成的过氧化氢（H2O2）生成系统相结合，建立了一个有效的单环氧化物生成系统（HX-XO-SOD-Cyt-c 系统）。利用该系统和 H2O2-Cyt-c 系统，我们证明了亚油酸生成单环氧化物是由于 Cyt-c 与 H2O2 反应形成羟基自由基，而非其他活性氧物种的形成。
