Schnitzer E, Pinchuk I, Fainaru M, Schafer Z, Lichtenberg D
Dept. of Physiology and Pharmacology, Belinson Hospital, Tel Aviv University, Sackler School of Medicine Ramat Aviv, Israel.
Biochem Biophys Res Commun. 1995 Nov 22;216(3):854-61. doi: 10.1006/bbrc.1995.2700.
In spite of the pathological significance of oxidation of plasma lipids, no method is currently available for the evaluation of the susceptibility of these lipids to oxidation in unfractionated plasma. Here we demonstrate that copper-induced oxidation of diluted plasma, in the presence of citrate can be monitored continuously by recording the absorbance at 245 nm. The kinetics of accumulation of oxidation products in unfractionated plasma is a sum of lipid oxidation products obtained in low and high density lipoproteins isolated from the same plasma. The kinetic profiles are reproducible and can be performed with plasma samples even after prolonged storage at 4 degrees C (up to two months) or after freezing and thawing of the plasma. Being simple and reproducible, yet correlating with the oxidizability of low and high density lipoproteins, this method can be used to evaluate the "oxidation resistance" of plasma lipids and thus serve as a standard index of the susceptibility of the plasma lipids of patients to oxidation-inflicted pathologies, including atherosclerosis.
尽管血浆脂质氧化具有病理意义，但目前尚无方法可用于评估这些脂质在未分级血浆中被氧化的易感性。在此我们证明，在柠檬酸盐存在的情况下，通过记录245nm处的吸光度，可以连续监测铜诱导的稀释血浆氧化。未分级血浆中氧化产物积累的动力学是从同一血浆中分离出的低密度和高密度脂蛋白中获得的脂质氧化产物的总和。动力学曲线是可重复的，即使血浆样品在4℃下长期保存（长达两个月）或经过冷冻和解冻后，也可对其进行检测。该方法简单且可重复，同时与低密度和高密度脂蛋白的氧化能力相关，可用于评估血浆脂质的“抗氧化性”，从而作为患者血浆脂质对氧化所致病理（包括动脉粥样硬化）易感性的标准指标。
