Lodén M, Boström P, Kneczke M
Apoteksbolaget Central Laboratory, Stockholm, Sweden.
Skin Pharmacol. 1995;8(4):173-8. doi: 10.1159/000211343.
Salicylic acid (SA) and urea are widely used in topical preparations. Using a simple tape stripping technique the effect on the binding forces within the stratum corneum and the skin absorption of SA and urea were studied. The degree of stratum corneum removal was recorded by measuring the transmission through the tape with a digital light-measuring instrument. With successive stripping of the skin the amount of tissue adhering to the tape decreased. Exposure of the upper arm to 2% SA for 6 h increased the skin material on the tape strips significantly. No significant increase was recorded after 3-hour exposure, or after exposure to 0.5% SA. Neither did the exposure to 10% urea for 3 or 6 h influence the amount of skin adhering to the tape significantly. Radiochemical analyses showed that the amount of SA and urea in each of the first 6 tape strips was about 5-15 micrograms/cm2. This technique provides a useful tool to evaluate the binding forces within the stratum corneum in relation to absorption of topically applied substances.
水杨酸（SA）和尿素广泛用于局部制剂。采用简单的胶带剥离技术，研究了其对角质层内结合力以及SA和尿素经皮吸收的影响。通过使用数字光测量仪器测量透过胶带的透射率来记录角质层去除的程度。随着皮肤的连续剥离，粘在胶带上的组织量减少。上臂暴露于2%的SA 6小时后，胶带条上的皮肤物质显著增加。暴露3小时后或暴露于0.5%的SA后，未记录到显著增加。暴露于10%的尿素3小时或6小时也未显著影响粘在胶带上的皮肤量。放射化学分析表明，前6条胶带中每条胶带的SA和尿素量约为5-15微克/平方厘米。该技术为评估角质层内与局部应用物质吸收相关的结合力提供了一种有用的工具。
