Imro M A, Castagneto C, Bosco O, Modena P, Lanza L, Puppo F, Filaci G, Indiveri F, Scudeletti M
Department of Internal Medicine, University of Genoa, Italy.
Cancer Immunol Immunother. 1995 Oct;41(4):210-6. doi: 10.1007/BF01516995.
In the present study T lymphocytes isolated from a metastatic lymph node (T-LNL) of a melanoma patient have been cloned. In the attempt to verify whether T-LNL may acquire in vitro functional activities in the absence of tumour-associated antigens, they were cloned utilizing allogenic lymphocytes as feeder cells. Nineteen clones generated from T-LNL proved to be CD4+ and, among these, five were able to kill autologous and allogeneic human melanoma cells in HLA-class-II-restricted way. On the basis of their cytokine production, these CD4+ cytolytic T-LNL clones were shown to belong to the Th0 subset and three of them expressed the V beta 17 chain of the T cell receptor. These results suggest the presence of melanoma-specific but functionally inactive lymphocytes with T cell receptor oligoclonality in the lymph node environment. These specific T cells may acquire in vitro the capacity to kill autologous and allogeneic tumours without any induction by autologous melanoma cells.
在本研究中，已克隆了从一名黑色素瘤患者的转移性淋巴结（T-LNL）中分离出的T淋巴细胞。为了验证T-LNL在没有肿瘤相关抗原的情况下是否能在体外获得功能活性，利用同种异体淋巴细胞作为饲养细胞对它们进行克隆。从T-LNL产生的19个克隆被证明是CD4+，其中5个能够以HLA-II类限制性方式杀伤自体和同种异体人黑色素瘤细胞。根据它们的细胞因子产生情况，这些CD4+溶细胞性T-LNL克隆被证明属于Th0亚群，其中3个表达T细胞受体的Vβ17链。这些结果表明，在淋巴结环境中存在具有T细胞受体寡克隆性的黑色素瘤特异性但功能无活性的淋巴细胞。这些特异性T细胞在体外可能获得杀伤自体和同种异体肿瘤的能力，而无需自体黑色素瘤细胞的任何诱导。
