Ambartsumian N S, Tarabykina S V, Grigorian M S, Tul'chinskiĭ E M, Hulgor I, Georgiev G P, Lukanidin E M
Danish Society for Cancer Research, Copenhagen, Denmark.
Genetika. 1995 Sep;31(9):1194-200.
Data on cloning of cDNA corresponding to human mts1 gene transcripts are presented. By comparing nucleotide sequences of the genomic DNA clone and cDNA of mts1, it was shown that human osteosarcoma OHS cells contain two alternative splice variants of mts1 transcripts. Alternative splicing occurs in the 5'-untranslated region of the mts1 pre-mRNA. Both splice variants, hu-mts1 and hu-mts1(var), demonstrate similar stability in the cells, and each contains one open reading frame for the MTS1 protein. However, the two types of transcripts are translated with different effectiveness. The level of transcription of mts1 splice variants in different normal and neoplastic tissues and cell lines varies significantly. The role of alternative splicing as the mechanism responsible for posttranscriptional regulation of mts1 gene expression is discussed.
本文展示了与人类mts1基因转录本相对应的cDNA克隆数据。通过比较mts1基因组DNA克隆和cDNA的核苷酸序列，发现人类骨肉瘤OHS细胞含有mts1转录本的两种可变剪接变体。可变剪接发生在mts1前体mRNA的5'-非翻译区。两种剪接变体，即hu-mts1和hu-mts1(var)，在细胞中表现出相似的稳定性，并且每种都包含一个MTS1蛋白的开放阅读框。然而，这两种类型的转录本翻译效率不同。mts1剪接变体在不同正常组织、肿瘤组织和细胞系中的转录水平差异显著。文中讨论了可变剪接作为负责mts1基因表达转录后调控机制的作用。
