Kanapina A Sh, Kanapin A A, Prozorov A A
Genetika. 1995 Sep;31(9):1201-9.
Nucleotide sequences of two minireplicon fragments of the p1414 cryptic plasmid of Bacillus subtilis were determined. The fragments corresponded to the region containing ori(+) and the gene coding for Rep protein. Comparing sequences of the fragments with corresponding sequences of other ss+ plasmids suggested that ori(+) of p1414 belongs to the family of endogenous cryptic B. subtilis plasmids, which form an individual, closely related subgroup in the group of ori(+) sequences of the pC194 type. It was found that the amino acid sequence of a conservative FLTLTV motif located, together with its flanking sequences, at the N ends of Rep proteins encoded by different ss+ plasmids, is similar to those of several transmembrane proteins and signal peptides. These results, together with computer data on predicting the secondary and tertiary structure of the N-terminal domain of the p1414 Rep protein, suggest that the domain can serve as a "membrane anchor" during plasmid replication.
测定了枯草芽孢杆菌p1414隐蔽质粒两个微型复制子片段的核苷酸序列。这些片段对应于含有ori(+)和编码Rep蛋白的基因的区域。将这些片段的序列与其他ss+质粒的相应序列进行比较表明，p1414的ori(+)属于枯草芽孢杆菌内源性隐蔽质粒家族，该家族在pC194型ori(+)序列组中形成一个单独的、密切相关的亚组。研究发现，位于不同ss+质粒编码的Rep蛋白N端的保守FLTLTV基序及其侧翼序列的氨基酸序列，与几种跨膜蛋白和信号肽的氨基酸序列相似。这些结果，连同关于预测p1414 Rep蛋白N端结构域二级和三级结构的计算机数据，表明该结构域在质粒复制过程中可作为“膜锚”。
