Poluéktova E U, Karandashova I V, Sapogova E Iu, Prozorov A A
Genetika. 1996 Nov;32(11):1498-503.
Peculiarities of DNA homology in a number of cryptic plasmids isolated from soil bacillary strains and related or identical to Bacillus subtilis were studied. Fragments generated after digestion of one of these plasmids, p1414, were employed as a probe for blot hybridization with identical fragments of other plasmids. The data obtained suggest that nearly all the studied plasmids possess a common homology site that occupies a significant plasmid portion. Regions of detectable and weak homology were located throughout this site. Moreover, plasmid p1414 was shown to carry a large site that lacks homology with plasmids belonging to two groups. Eventually, one of these plasmids demonstrates complete lack of homology with the remaining plasmids.
研究了从土壤杆菌菌株中分离出的一些与枯草芽孢杆菌相关或相同的隐蔽质粒的DNA同源性特点。其中一个质粒p1414消化后产生的片段被用作与其他质粒的相同片段进行印迹杂交的探针。获得的数据表明，几乎所有研究的质粒都有一个共同的同源位点，该位点占据了质粒的很大一部分。在这个位点上分布着可检测到的同源区域和弱同源区域。此外，质粒p1414显示携带一个与两组质粒都缺乏同源性的大片段。最终，其中一个质粒与其余质粒完全缺乏同源性。
