McCluggage W G, Roddy S, Whiteside C, Burton J, McBride H, Maxwell P, Bharucha H
Department of Pathology, Royal Group of Hospitals Trust, Belfast.
J Clin Pathol. 1995 Sep;48(9):840-4. doi: 10.1136/jcp.48.9.840.
To investigate (1) whether adequate immunohistochemical staining can be achieved on sections cut from plastic embedded bone marrow trephine biopsy specimens after microwave heating in citrate buffer; and (2) whether this immunohistochemical staining is comparable with that achieved on routine sections cut from paraffin wax embedded trephine biopsy specimens after decalcification procedures.
Sixty five consecutive bone marrow trephine biopsy specimens of more than 1 cm in length were divided transversely into two equal parts. One part was processed in paraffin wax followed by decalcification. The other part was embedded in the epoxyresin Polarbed 812 followed by the cutting of 1 micron sections. Both parts underwent immunohistochemical staining by an identical panel of antibodies. With Polarbed 812 plastic embedded sections, microwave heating in citrate buffer was undertaken before the application of antisera.
On sections cut from plastic embedded material, immunohistochemical staining was generally satisfactory, easy to interpret and comparable with that achieved with paraffin wax embedded material. Exceptions were antibodies to neutrophil elastase and CD61 where immunostaining was consistently negative on plastic embedded sections. Immunohistochemical staining for CD20 was consistently more reliable on plastic embedded sections.
The results provide evidence that, with few exceptions, satisfactory immunohistochemical staining is possible on plastic embedded bone marrow trephine biopsy specimens after microwave heating in citrate buffer. This, combined with the advantage of superior cellular morphology with semi-thin (1 micron) sections of plastic embedded material, make such embedding procedures the preferred method for the processing of bone marrow trephine biopsy specimens.
研究（1）经柠檬酸盐缓冲液微波加热后的塑料包埋骨髓活检组织切片能否获得充分的免疫组化染色；（2）这种免疫组化染色与经脱钙处理后的石蜡包埋活检组织常规切片的免疫组化染色是否具有可比性。
选取65例连续的长度超过1cm的骨髓活检组织标本，横向等分为两部分。一部分进行石蜡包埋，随后脱钙。另一部分包埋于环氧树脂Polarbed 812中，然后切成1微米厚的切片。两部分均用同一组抗体进行免疫组化染色。对于Polarbed 812塑料包埋切片，在应用抗血清之前先在柠檬酸盐缓冲液中进行微波加热。
塑料包埋材料切片的免疫组化染色总体上令人满意，易于解读，且与石蜡包埋材料的染色效果相当。嗜中性粒细胞弹性蛋白酶抗体和CD61抗体除外，其在塑料包埋切片上的免疫染色始终为阴性。CD20的免疫组化染色在塑料包埋切片上始终更可靠。
结果表明，除少数例外情况，经柠檬酸盐缓冲液微波加热后的塑料包埋骨髓活检组织切片能够获得令人满意的免疫组化染色。这与塑料包埋材料的半薄（1微米）切片具有更好的细胞形态学优势相结合，使这种包埋方法成为处理骨髓活检组织标本的首选方法。
