Igarashi M, Nakayama S, Jones E, Shimada H
Drug Research Center, Developmental Research Institute, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan.
J Toxicol Sci. 1995 Oct;20 Suppl 1:125-31. doi: 10.2131/jts.20.supplementi_125.
A new non-ionic isotonic contrast medium, iodixanol (a diastereomeric mixture of 5,5'-[(2-hydroxytrimethylene) bis (acetylimino)] bis [N, N'-bis (2,3-dihydroxypropyl)-2,4,6-triiodo-1,3-benzene-dicarboxamide]) was studied for mutagenicity by using the Ames method, in vitro cytogenetics and micronucleus test. Iodixanol had no mutagenic effect on S. typhimurium (TA1535, TA1537, TA1538, TA98 and TA100) or E. coli (WP2uvrA) in the reverse mutation assay with or without metabolic activations. In the cytogenetic study, iodixanol had no effect on the Chinese hamster cells with or without metabolic activations. Intravenous injections of iodixanol for two times at a dose of 800, 1,600 or 3,200 mgI/kg in the mouse micronucleus test did not increase the incidence of micronucleated polychromatic erythrocytes. These results show that iodixanol has no mutagenic potential.
一种新型非离子等渗造影剂碘克沙醇（5,5'-[(2-羟基亚丙基)双(乙酰亚氨基)]双[N,N'-双(2,3-二羟基丙基)-2,4,6-三碘-1,3-苯二甲酰胺]的非对映体混合物），通过艾姆斯试验、体外细胞遗传学和微核试验研究其致突变性。在有或无代谢活化的回复突变试验中，碘克沙醇对鼠伤寒沙门氏菌（TA1535、TA1537、TA1538、TA98和TA100）或大肠杆菌（WP2uvrA）均无致突变作用。在细胞遗传学研究中，碘克沙醇在有或无代谢活化的情况下对中国仓鼠细胞均无影响。在小鼠微核试验中，以800、1600或3200 mgI/kg的剂量静脉注射碘克沙醇两次，并未增加多染性红细胞微核的发生率。这些结果表明碘克沙醇无致突变潜力。
