Chen L T, Huang M S, Wang Y L, Liu C S, Jan C M
Department of Internal Medicine, Kaohsiung Medical College, Taiwan, Republic of China.
Gaoxiong Yi Xue Ke Xue Za Zhi. 1995 Nov;11(11):615-20.
Argyrophilic nucleolar organizer regions (AgNOR) can reflect the activity of cellular proliferation. The purpose of this study is to evaluate the potential value of AgNOR in differentiating benign from malignant colon epithelial neoplasms, and to determine the correlation between the nucleus AgNOR and the grade of colonic adenocarcinomas.
In this study, AgNOR technique was applied to 61 paraffin embedded sections of colorectal tissue including normal mucosa (n = 10), adenomatous polyp (n = 16), and adenocarcinoma (n = 35).
The mean +/- standard error (SE) numbers of AgNOR dots per nucleus of normal mucosa, adenomatous polyp, and adenocarcinoma were 2.17 +/- 0.07 (n = 10) 3.89 +/- 0.10 (n = 16) and 5.52 +/- 0.10 (n = 35), respectively (p < 0.00001). In addition, the mean numbers of AgNOR dots per nucleus of well differentiated (WD) adenocarcinoma (n = 14), moderately differentiated (MD) adenocarcinoma (n = 11) and poorly differentiated (PD) adenocarcinoma (n = 10) were 5.20 +/- 0.12, 5.81 +/- 0.20, 5.67 +/- 0.15, respectively. MD and PD tumor had significantly higher AgNOR count than that of WD tumor (p < 0.05). However, there was no significant difference between MD and PD colorectal adenocarcinoma.
AgNOR method is a simple, rapid method in diagnosis of colorectal tumors, and it provides a useful adjunct to histopathology in the diagnosis of colorectal tumors.
嗜银核仁组成区（AgNOR）可反映细胞增殖活性。本研究旨在评估AgNOR在鉴别结肠上皮良性与恶性肿瘤中的潜在价值，并确定细胞核AgNOR与结肠腺癌分级之间的相关性。
本研究将AgNOR技术应用于61例结直肠组织石蜡包埋切片，包括正常黏膜（n = 10）、腺瘤性息肉（n = 16）和腺癌（n = 35）。
正常黏膜、腺瘤性息肉和腺癌每细胞核AgNOR点的平均±标准误（SE）数分别为2.17±0.07（n = 10）、3.89±0.10（n = 16）和5.52±0.10（n = 35）（p < 0.00001）。此外，高分化（WD）腺癌（n = 14）、中分化（MD）腺癌（n = 11）和低分化（PD）腺癌（n = 10）每细胞核AgNOR点的平均数分别为5.20±0.12、5.81±0.20、5.67±0.15。MD和PD肿瘤的AgNOR计数显著高于WD肿瘤（p < 0.05）。然而，MD和PD结直肠腺癌之间无显著差异。
AgNOR方法是一种诊断结直肠肿瘤的简单、快速方法，为结直肠肿瘤的组织病理学诊断提供了有用的辅助手段。
