Arora H L, Arora N, Solanki R L
Department of Pathology, S.P. Medical College, Bikaner.
Indian J Pathol Microbiol. 1996 Oct;39(4):257-63.
Argyrophilic nucleolar organizer region (AgNOR) staining was employed on 51 apparently normal representative soft tissues, 53 benign soft tissues tumors and 52 malignant soft tissue tumors with an aim to study the sensitivity and specificity of method in differentiating between the benign and malignant soft tissue tumors. The mean AgNORs count in apparently normal fibrous tissue was 1.02, whereas it was 0.94 in adipose tissue, 1.14 in smooth muscle tissue, 1.115 in skeletal muscle tissue, 1.025 in blood vessels endothelial lining cells and 1.04 in nerve tissue. The mean AgNOR count was found to be higher in benign soft tissue tumors as compared to respective apparently normal soft tissue and was found to be statistically significant. The mean AgNOR count in soft tissue sarcomas was found to be higher as compared to both apparently normal soft tissue and benign soft tissue tumors. An increase AgNOR score in both benign and malignant soft tissue tumors as compared to apparently normal soft tissue indicates high proliferative activity. The neurofibrosarcoma showed low AgNOR count as compared to other soft tissues sarcomas. The fibrohistiocytic sarcoma, leiomyosarcoma and angiosarcoma showed a mean AgNOR score of 4 or more than four. The mean AgNOR score was found to increase with high grade of the tumor. The AgNOR staining is simple and useful method in estimating tumor cell proliferation thereby differentiating normal soft tissue from non-neoplastic proliferative growth, benign and malignant soft tissue tumors. It may help in differentiating fibromatosis from fibrosarcoma, dermatofibrosarcoma protuberans of low grade malignancy from high grade malignant fibrous histiocytoma and benign hemangiopericytoma from malignant hemangiopericytoma.
对51例外观正常的代表性软组织、53例良性软组织肿瘤和52例恶性软组织肿瘤进行嗜银核仁组织区（AgNOR）染色，旨在研究该方法在鉴别良性和恶性软组织肿瘤方面的敏感性和特异性。外观正常的纤维组织中AgNORs计数的平均值为1.02，而脂肪组织中为0.94，平滑肌组织中为1.14，骨骼肌组织中为1.115，血管内皮衬里细胞中为1.025，神经组织中为1.04。结果发现，良性软组织肿瘤中的AgNOR平均计数高于各自外观正常的软组织，且具有统计学意义。与外观正常的软组织和良性软组织肿瘤相比，软组织肉瘤中的AgNOR平均计数更高。与外观正常的软组织相比，良性和恶性软组织肿瘤中AgNOR评分的增加表明增殖活性较高。与其他软组织肉瘤相比，神经纤维肉瘤的AgNOR计数较低。纤维组织细胞肉瘤、平滑肌肉瘤和血管肉瘤的AgNOR平均评分为4或更高。发现AgNOR平均评分随肿瘤分级升高而增加。AgNOR染色是一种简单且有用的方法，可用于评估肿瘤细胞增殖，从而区分正常软组织与非肿瘤性增殖性生长、良性和恶性软组织肿瘤。它可能有助于鉴别纤维瘤病与纤维肉瘤、低级别恶性的隆突性皮肤纤维肉瘤与高级别恶性纤维组织细胞瘤，以及良性血管外皮细胞瘤与恶性血管外皮细胞瘤。
