Koshida K, Uchibayashi T, Yamamoto H, Yokoyama K, Hirano K
Department of Urology, School of Medicine, Kanazawa University, Japan.
J Urol. 1996 Jan;155(1):337-41.
To evaluate the ability of monoclonal antibodies against alkaline phosphatase (AL-P) for tumor immunolocalization in nude mice.
Two monoclonal antibodies against placental alkaline phosphatase (PLAP) and liver alkaline phosphatase (LAP) were intravenously administered to nude mice bearing AL-P producing HeLa Hep 2 tumors. Sera and tumors from 65 patients with testicular germ cell tumors were examined for PLAP levels.
Levels of PLAP or LAP in the HeLa xenografts were shown to be comparable to levels of PLAP in seminomas, including lymph node metastases, or nonseminomas, respectively. A biodistribution study showed that the percentage of the injected dose of I-125 labelled anti-PLAP in the xenografts was rather constant at around 7% until 7 days after injection, while the percentage of the injected dose of I-125 labelled anti-LAP MAb decreased with time as in other tissues which do not contain significant amounts of PLAP or LAP. On scintiscan, the xenografts in nude mice were distinctly visualized at 7 days after injection of anti-PLAP MAb.
These data may support the potential use of the anti-PLAP MAb for the immunodetection of lymph node metastases of seminoma.
评估抗碱性磷酸酶（AL-P）单克隆抗体在裸鼠体内进行肿瘤免疫定位的能力。
将两种分别针对胎盘碱性磷酸酶（PLAP）和肝碱性磷酸酶（LAP）的单克隆抗体静脉注射给携带产生AL-P的HeLa Hep 2肿瘤的裸鼠。检测65例睾丸生殖细胞肿瘤患者的血清和肿瘤组织中的PLAP水平。
HeLa异种移植瘤中PLAP或LAP的水平分别与精原细胞瘤（包括淋巴结转移灶）或非精原细胞瘤中PLAP的水平相当。生物分布研究表明，直到注射后7天，I-125标记的抗PLAP在异种移植瘤中的注射剂量百分比相当稳定，约为7%，而I-125标记的抗LAP单克隆抗体的注射剂量百分比随时间下降，与其他不含大量PLAP或LAP的组织情况相同。在闪烁扫描中，注射抗PLAP单克隆抗体7天后，裸鼠体内的异种移植瘤清晰可见。
这些数据可能支持抗PLAP单克隆抗体在精原细胞瘤淋巴结转移免疫检测中的潜在应用。
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