天然干扰素增强人精原细胞瘤异种移植物中胎盘碱性磷酸酶的表达。

Natural interferon enhances expression of placental alkaline phosphatase in human seminoma xenograft.

作者信息

Imao T, Koshida K, Konaka H, Uchibayashi T, Yokoyama K, Hirano K, Namiki M

机构信息

Department of Urology, School of Medicine, Kanazawa University, Japan.

出版信息

Urol Res. 1998;26(6):377-82. doi: 10.1007/s002400050072.

DOI:10.1007/s002400050072

PMID:9879816

Abstract

The purpose of this study was to investigate the effect of natural interferon (IFN) on the expression of placental alkaline phosphatase (PLAP) in a human seminoma xenograft in severe combined immunodeficient mice. Mice were injected intramuscularly with 3x10(5) U/mouse of IFN, twice a day, for five consecutive days. A significant increase in PLAP level of the xenografts followed IFN treatment. A radiolabeled anti-PLAP monoclonal antibody (MAb) was intravenously injected on the first day of IFN administration in order to determine if IFN has the potential to enhance the efficacy of an anti-PLAP MAb in the detection of seminoma. Enhanced retention of the anti-PLAP MAb was observed at 7 and 11 days after MAb administration. Thus, IFN treatment appears to have some effect on the efficacy of the anti-PLAP MAb in the detection of seminoma xenografts.

摘要

本研究的目的是探讨天然干扰素（IFN）对严重联合免疫缺陷小鼠体内人精原细胞瘤异种移植物中胎盘碱性磷酸酶（PLAP）表达的影响。小鼠连续五天每天两次肌肉注射3×10⁵ U/只的IFN。IFN治疗后，异种移植物中PLAP水平显著升高。在给予IFN的第一天静脉注射放射性标记的抗PLAP单克隆抗体（MAb），以确定IFN是否有增强抗PLAP MAb在精原细胞瘤检测中的疗效的潜力。在给予MAb后第7天和第11天观察到抗PLAP MAb的滞留增强。因此，IFN治疗似乎对抗PLAP MAb在精原细胞瘤异种移植物检测中的疗效有一定影响。