精原细胞瘤中碱性磷酸酶的同工酶。一项免疫组织化学和生物化学研究。

Isoenzymes of alkaline phosphatases in seminomas. An immunohistochemical and biochemical study.

作者信息

Brehmer-Andersson E, Ljungdahl-Ståhle E, Koshida K, Yamamoto H, Stigbrand T, Wahren B

机构信息

Department of Pathology, Södersjukhuset, Stockholm, Sweden.

出版信息

APMIS. 1990 Nov;98(11):977-82. doi: 10.1111/j.1699-0463.1990.tb05023.x.

DOI:10.1111/j.1699-0463.1990.tb05023.x

PMID:2248772

Abstract

Placental alkaline phosphatase (the PLAP-like isoenzyme) and liver alkaline phosphatase (LAP) were demonstrated immunohistochemically by use of monoclonal antibodies in the tumor cells of twelve seminomas and one seminoma metastasis. Intestinal alkaline phosphatase (IAP) was not found. The PLAP-like and LAP enzymes showed high catalytic activities compared to normal testis. This is the first occasion that LAP has been demonstrated by immunochemistry in seminoma cells. The results suggest that demonstration of these tumor enzymes may be useful markers for seminomas in histopathological specimens.

摘要

运用单克隆抗体通过免疫组织化学方法在12例精原细胞瘤及1例精原细胞瘤转移灶的肿瘤细胞中检测胎盘碱性磷酸酶（类胎盘碱性磷酸酶同工酶）和肝碱性磷酸酶（LAP）。未发现肠碱性磷酸酶（IAP）。与正常睾丸相比，类胎盘碱性磷酸酶和肝碱性磷酸酶显示出较高的催化活性。这是首次通过免疫化学方法在精原细胞瘤细胞中检测到LAP。结果表明，在组织病理学标本中检测这些肿瘤酶可能是精原细胞瘤有用的标志物。