Sitsapesan R, Montgomery R A, Williams A J
Department of Cardiac Medicine, National Heart and Lung Institute, London, UK.
Pflugers Arch. 1995 Aug;430(4):584-9. doi: 10.1007/BF00373896.
We have developed a method of rapidly changing the solutions on one side of a planar phospholipid bilayer. Bilayers can be painted on glass pipettes of tip diameter > or = 50 microns. By modifying an established method for rapid exchange of solutions bathing excised membrane patches, solution changes can be made at the bilayer within 10 ms. After incorporation of channels into the bilayer, the bilayer is moved into one of two parallel streams of solution flowing from a length of double-barrelled glass theta tubing. Activation of a solenoid system rapidly moves the theta tubing so that the bilayer is in the flow of the adjacent solution. For various reasons, the single-channel gating mechanisms of many channels are studied in planar bilayer systems. The conventional bilayer technique only allows for steady-state single-channel gating to be monitored. This novel method now allows the effects of rapid changes in modulators of channels incorporated into planar phospholipid bilayers to be measured.
我们开发了一种可快速更换平面磷脂双分子层一侧溶液的方法。双分子层可涂覆在尖端直径≥50微米的玻璃移液管上。通过改进一种用于快速更换浸泡切除膜片溶液的既定方法，可在10毫秒内在双分子层处实现溶液更换。将通道整合到双分子层后，将双分子层移入从一段双管玻璃θ形管流出的两股平行溶液流之一中。激活一个螺线管系统可快速移动θ形管，使双分子层处于相邻溶液的流动中。由于各种原因，许多通道的单通道门控机制是在平面双分子层系统中进行研究的。传统的双分子层技术仅允许监测稳态单通道门控。这种新方法现在能够测量整合到平面磷脂双分子层中的通道调节剂快速变化的影响。
