Aoyagi M, Yamamoto M, Wakimoto H, Azuma H, Hirakawa K, Yamamoto K
Department of Neurosurgery, Tokyo Medical and Dental University, Japan.
Stroke. 1995 Dec;26(12):2328-31; discussion 2331-2. doi: 10.1161/01.str.26.12.2328.
Ki-67 immunohistochemistry has been shown to be useful for paraffin sections from human species after wet heating. We applied Ki-67 immunohistochemistry to rabbit arteries after balloon denudation and compared the results with proliferating cell nuclear antigen (PCNA) immunohistochemistry.
Monoclonal antibodies MIB-1 for Ki-67 and PC-10 for PCNA were used to detect replicative smooth muscle cells in rabbit carotid arteries during a period of 4 weeks after balloon endothelial denudation.
We demonstrated clear immunoreactivities for Ki-67 in paraffin sections of rabbit arteries after hydrated autoclaving. Ki-67-positive smooth muscle cells appeared throughout the thickened intima 1 week after endothelial denudation. At 2 weeks, Ki-67-positive cells were confined to the surface layer of the intima. PCNA-positive cells appeared in almost the same location by 2 weeks after endothelial denudation but were significantly greater in number than Ki-67-positive cells. PCNA-positive cells remained in the surface layer of the intima 4 weeks after endothelial denudation, while Ki-67-positive cells had almost disappeared from the intima.
Our results indicate that Ki-67 immunohistochemistry using MIB-1 monoclonal antibody provides a powerful tool, even in rabbit species, for the detection of replicative smooth muscle cells during the repair of arterial injury and that it detects replicative cells more accurately than PCNA immunohistochemistry.
已证实Ki-67免疫组织化学对经湿热处理后的人类石蜡切片有用。我们将Ki-67免疫组织化学应用于球囊剥脱术后的兔动脉，并将结果与增殖细胞核抗原（PCNA）免疫组织化学进行比较。
使用针对Ki-67的单克隆抗体MIB-1和针对PCNA的PC-10来检测兔颈动脉球囊内皮剥脱后4周内复制性平滑肌细胞。
我们证实在水合高压灭菌后的兔动脉石蜡切片中Ki-67有明显的免疫反应性。内皮剥脱1周后，Ki-67阳性平滑肌细胞出现在增厚的内膜各处。2周时，Ki-67阳性细胞局限于内膜表层。内皮剥脱2周后，PCNA阳性细胞出现在几乎相同的位置，但数量明显多于Ki-67阳性细胞。内皮剥脱4周后，PCNA阳性细胞仍留在内膜表层，而Ki-67阳性细胞几乎已从内膜消失。
我们的结果表明，使用MIB-1单克隆抗体的Ki-67免疫组织化学即使在兔类中也是检测动脉损伤修复过程中复制性平滑肌细胞的有力工具，并且它比PCNA免疫组织化学更准确地检测复制性细胞。
